12 LABORATORY MANUAL OF MICROBIOLOGY 



In order to prevent decomposition of the glucose, it is well to 

 sterilize the calcium carbonate separately. Tube in deep layers. 

 For a solid medium add 15 grams of agar to each liter. 



Medium 12 



Winogradsky's Glucose-peptone Agar 



Agar 15.0 gm. 



Peptone 5.0 gm. 



Beef extract 3.0 gm. 



Glucose 5.0 gm. 



Tap water 1,000.0 cc. 



Reaction, pH 7.0. Deep tubes. 



Medium 13 

 Peptone-mannitol Solution for Anaerobic Bacteria^ 



Peptone 2.5 gm. 



Beef extract 2.0 gm. 



Mannitol or glucose 10.0 gm. 



Manganese sulphate (MnS04-4H20) 0.004 gm. 



Tap water 1,000.0 cc. 



1 Truffaut, G. et N. Bezssonoff, Compt. rend. Acad. Sci., 173: 868, 1921. 



Adjust the reaction to pH 7.0. 



MEDIA FOR FUNGI 



Medium 14 



Raulin's Solution 



Ammonium nitrate (NH4NO3) 4.0 gm. 



Ammonium phosphate ((NH4)2HP04) 0.6 gm. 



Ammonium sulphate ((NH4)2S04) . 25 gm. 



Potassium carbonate (K2CO3) 0.6 gm. 



Magnesium carbonate (MgCOs) 0.4 gm. 



Zinc sulphate (ZnS04) 0.07 gm. 



Ferrous sulphate (FeS04-7H20) . 07 gm. 



Potassium sihcate (K2Si03) . 07 gm. 



Tartaric acid 4.0 gm. 



Sucrose 70 . gm. 



Distilled water 1,500.0 cc. 



This medium is given here due to its historical significance. 

 The cultivation of fungi can be carried out successfully on a much 

 simpler medium. 



