CULTURE MEDIA 13 



Medium 15 

 Ammonium Nitrate-sucrose Solution 



Ammonium nitrate (NH4NO3) 3.0 gm. 



Monopotassium phosphate (KH2PO4) 1.0 gm. 



Magnesium sulphate (MgS04-7H20) 1.0 gm. 



Ferrous sulphate (FeS04-7H20) trace 



Sucrose 50 . gm. 



Distilled water 1,000 . cc. 



Medium 16 



Sodium Nitrate-sucrose Solution (Czapek's)^ 



Sodium nitrate (NaNOs) 2.0 gm. 



Monopotassium phosphate (KH2PO4) 1 .00 gm. 



Potassium chloride (KCl) . 50 gm. 



Magnesium sulphate (MgS04-7H20) . 50 gm. 



Ferrous sulphate (FeS04 -71120 0.01 gm. 



Sucrose 30 . 00 gm. 



Distilled water 1,000.00 cc. 



1 CzAPEK, F., Beitrage z. chem. Phijsiol. u. Path., I: 538-560, 1901. 



To prepare an agar medium, add 15 grams of agar to 1,000 cubic 

 centimeters of solution. Dissolve and filter. The reaction is 

 left unadjusted. 



Medium 17 



Asparagin-glucose Agari 



Agar 15.0 gm. 



Glucose 10 . gm. 



Asparagin 0.5 gm. 



Dipotassium phosphate (K2HPO4) 0.5 gm. 



Distilled water 1,000 . cc. 



1 Krainsky, a., Centr. Bakt. II Abt., 41: 649-688, 1914. 

 This medium is also used for the cultivation of actinomyces. 



Medium 18 

 Peptone-glucose Acid Agar^ 



Agar 25.0 gm. 



Monopotassium phosphate (KH2PO4) 1.0 gm. 



Magnesium sulphate (MgS04-7H20) 0.5 gm. 



Peptone 5.0 gm. 



Glucose 10.0 gm. 



Water 1,000 . cc. 



1 Waksman, S. a., /. BacL, 7: 339-341, 1922. 



Reaction pH 3.8 to 4.0. 



