CULTURE MEDIA 15 



Medium 21 



Clover-sucrose Agar 



Agar 25 . gm. 



Clover (green tops) 500 . gm. 



Sucrose 2.0 gm. 



Potassium nitrate (KNO3) 0.5 gm. 



Tap water 1,000.0 cc. 



Extract the clover tissue by heating for 1 hour in the steamer; 

 filter and add the other ingredients. Add 1 to 2 drops of concen- 

 trated lactic acid to each tube of the medium just before pouring 

 plates. 



Reaction approximately pH 3.5. 



Medium 22 

 Peptone-malt Agar^ 



Agar 20 . gm. 



Peptone 1.0 gm. 



Glucose 20.0 gm. 



Malt extract (desiccated) 20 . gm. 



Tap water 1,000.0 cc. 



1 PovAH, A. H. W., Torrey Bot. Club. Bull, 44: 241, 287, 1917. 

 This medium is used for the cultivation of Mucorales. 



Medium 23 

 Ammonium Nitrate Lactic Acid Solution^ 



Ammonium nitrate (NH4NO3) 10.0 gm. 



Dipotassium- phosphate (K2HPO4) 5.0 gm. 



Magnesium sulphate (MgS04-7H20) 1.0 gm. 



Lactic acid (85 per cent) 2.0 gm. 



Tap water 1,000.0 cc. 



2v. TuBEUF, C, Centr. Bakt., II Abt., 9: 127-135, 1902. 



Fifty-cubic centimeter portions of this solution are added 

 to flasks containing 10 grams of filter paper. This medium is 

 used for the cultivation of wood-destroying fungi. 



