CULTURE MEDIA 19 



Medium 35 

 Sodium Asparaginate-glycerol Agar^ 



Agar 15.0 gm. 



Glycerol 10.0 cc. 



Dipotassium phosphate (K2HPO4) 1.0 gm. 



Sodium asparaginate 1.0 gm. 



Water 1,000.0 cc. 



1 Conn, H. J., N. Y. Agr. Expt. Sta., Tech. Bull 60, 1917. 



Reaction approximately pH 7.0. 



Medium 36 



Ammonium Sulphate-starch Agar^ 



Agar 10.0 gm. 



Starch (soluble) 10 . gm. 



Ammonium sulphate ((NH4)2S04) 2.0 gm. 



Dipotassium phosphate (K2HPO4) 1.0 gm. 



Magnesium sulphate (MgS04-7H20) 1.0 gm. 



Sodium chloride 1.0 gm. 



Calcium carbonate (CaCOs) 3.0 gm. 



Tap water 1,000.0 cc. 



2 McBeth, I. G., and F. M. Scales, U. S. Dept. Agr., Bur. PI. Ind. Bull. 

 266, 1913. 



Mix the starch with a little cold water and stir well before 

 adding the salts. 



MEDIA FOR PROTOZOA 



Medium 37 



Nutrient or Beef Extract Agar^ 



Agar 15.0 gm. 



Beef extract 3.0 gm. 



Peptone 10.0 gm. 



Sodium chloride (NaCl) 5.0 gm. 



Distilled water 1,000.0 cc. 



3 Sandon, H., and D. W. Cutler, /. Linn. Soc. Zool, 38 : 1, 1924. 



Adjust the reaction to pH 7.0. 



Medium 38 

 Hay Infusion 



Meadow hay (finely chopped) 50 . gm. 



Tap water 1,000.0 cc. 



Boil for 2 hours and allow to stand over night. Filter. 



