20 LABORATORY MANUAL OF MICROBIOLOGY 



Medium 39 



Ammonium Lactate Solution 



Ammonium lactate 0.1 gm. 



Ammonium chloride (NH^Cl) 0.3 gm. 



Potassium chloride (KCl) 0.3 gm. 



Magnesium sulphate (MgS04-7H20) 0.001 gm. 



Disodium phosphate (Na2HP04) 0.01 gm. 



Calcium chloride (CaCU) 0.2 gm. 



Glucose 0.4 gm. 



Distilled water 1,000 . cc. 



Medium 40 



Mannitol Soil Extract 



Soil extract (stock) 100 . cc. 



Mannitol 10.0 gm. 



Dipotassium phosphate (K2HPO4) 0.5 gm. 



Tap water 900 . cc. 



Medium 41 

 Mannitol-phosphate Solution^ 



Mannitol (C6H8(OH)6) 10.0 gm. 



Magnesium sulphate (MgS04-7H20) 0.2 gm. 



Monopotassium phosphate (KH2PO4) 0.2 gm. 



Sodium chloride (NaCl) . 2 gm. 



Calcium sulphate (CaS04-2H20) 0.1 gm. 



Calcium carbonate (CaCOs) 5.0 gm. 



Distilled water 1,000.0 cc. 



1 AsHBY, S. F., J. Agr. Sci., 2: 38, 1907. 



Dissolve the phosphate separately in a little water and make 

 the solution neutral to phenolphthalein with A^/1 NaOH; then 

 add to the other ingredients. For a solid medium add 15 grams 

 of agar to each liter. 



MEDIA FOR ALG^ 



Medium 42 

 Calcium Nitrate Solution (Detmer's) 



Calcium nitrate (Ca(N03)2) 10 gm. 



Potassium chloride (KCl) 0. 25 gm. 



Magnesium sulphate (MgS04-7H20) . 25 gm. 



Monopotassium phosphate (KH2PO4) . 25 gm. 



Tap water 1,000.0 cc. 



