26 LABORATORY MANUAL OF MICROBIOLOGY 



Medium 58 

 Potassium Nitrate-filter Papers 



Dipotassium phosphate (K2HPO4) 0.5 gm. 



Potassium nitrate (KNO3) 2.5 gm. 



Filter paper in strips 20 . gm. 



Tap water 1,000 . cc. 



I Van Iterson, Centr. Bakt., II Abt., 11: 689, 1904. 



Medium 59 

 Potassium Nitrate-thiosulphate Solution^ 



Sodium thiosulphate (Na2S203-5H20) 5.0 gm. 



Potassium nitrate (KNO3) 5.0 gm. 



Sodium bicarbonate (NaHCOs) 1.0 gm. 



Dipotassium phosphate (K2HPO4) 0.2 gm. 



Magnesium chloride (MgCU'OHoO) 0.1 gm. 



Calcium chloride (CaCl2-6H20) trace 



Ferric chloride (FeCl3-6H20) trace 



Distilled water 1,000.0 cc. 



2L1ESKE, R., Ber. d. Deutsch. Bot. Gessell, 30: 12-22, 1912. 



MEDIA FOR THE REDUCTION OF SULPHATES AND OTHER 

 SULPHUR COMPOUNDS 



Medium 60 



ASPARAGIN-SODIUM LACTATE SOLUTION^ 



Asparagin (C4H8N2O3H2O) 1.0 gm. 



Sodium lactate (NaC3H503) 5.0 gm. 



Dipotassium phosphate (K2HPO4) 0.5 gm. 



Magnesium sulphate (MgS04-7H20) 1.0 gm. 



Ferrous sulphate (FeS04-7H20) trace 



Tap water 1,000.0 cc. 



3 Van Delden, A., Centr. Bakt., II Abt., 11: 83, 1904. 



Medium 61 



Sodium Lactate-ammonium Sulphate Solution 



Ammonium sulphate ((NH4)2S04) 2.0 gm. 



Dipotassium phosphate (K2HPO4) 0.5 gm. 



Sodium lactate (NaCsHsOs) 5.0 gm. 



Ferrous sulphate (FeS04-7H20) trace 



Tap water 1,000.0 cc. 



To prepare a solid medium add the above ingredients to 15 

 per cent gelatin. Heat the medium in a steamer until the 



