CULTURE MEDIA 31 



Medium 72 

 Ammonium Chloride Solution for Hydrogen Bacteria* 



Sodium bicarbonate (NaHCOs) 1.0 gm. 



Ammonium chloride (NH4CI) 1.0 gm. 



Monopotassium phosphate (KH2PO4) 0.5 gm. 



Ferrous carbonate (FeC03-H20) 0.5 gm. 



Magnesium sulphate (MgS04-7H20) 0.1 gm. 



Sodium chloride (NaCl) 0.1 gm. 



Distilled water 1,000.0 cc. 



pH = 7.1 - 7.2. 



' RuHLAND, W., Jahrh. Wissen. BoL, 63: 321, 1924. 



Use freshly prepared NaHCOs. Iron must be added in the 

 form of ferrous carbonate. A sterile solution of the iron salt in 

 water containing CO2 is added to the sterile medium. The 

 culture is grown in an atmosphere containing hydrogen and 

 carbon dioxide. 



Medium 73 



Magnesium Ammonium Phosphate Solution for Methane Bacteria^ 



Magnesium ammonium phosphate (MgNH4- 



P04-6HoO) 0.1 gm. 



Dipotassium phosphate (K2HPO4) .05 gm. 



Calcium sulphate (CaS04-2H20) . 01 gm. 



Distilled water 1,000 cc. 



2 Sohngen, N. L., Bot. Centr., 105: 371-372, 1907. 



The cultures are placed in an atmosphere consisting of 1 part 

 methane (CH4) and 2 parts air. 



Silica gel or washed agar containing the above nutrients can 

 be conveniently used as solid media. 



MEDIA FOR IRON AND MANGANESE OXIDIZING BACTERIA 



Medium 74 



Iron-ammonium Sulphate Solution^ 



Ammonium sulphate ((NH4)2S04) 1-5 gm. 



Potassium chloride (KCl) . 05 gm. 



Magnesium sulphate (MgS04-7H20) 0.05 gm. 



Dipotassium phosphate (K2HPO4) .05 gm. 



Calcium nitrate (Ca(N03)2) 0.01 gm. 



Distilled water 1,000.0 cc. 



sLieske, R., Centr. Bakt., II Abt., 49: 413-425, 1919. 



