38 LABORATORY MANUAL OF MICROBIOLOGY 



cellulose in the solution up to 0.5 per cent by allowing to settle a 

 few days and siphoning off the clear solution or by evaporating. 

 Add the nutrient salts desired, together with 1 per cent of 

 thoroughly washed agar; heat in autoclave or boil until the agar 

 is dissolved; tube and sterilize in the usual way. 



Medium 88 



Cellulose-peptone Agar 



Agar (washed) 15.0 gm. 



Cellulose ! 2.5 gm. 



Peptone . 50 gm. 



Dipotassium phosphate (K2HPO4) 0.2 gm. 



Magnesium sulphate (MgS04-7H20) 0.2 gm. 



Potassium carbonate (K2CO3) 0.4 gm. 



Calcium chloride (CaCU) fused 0.02 gm. 



Ferric sulphate (Fe2(S04)3-9H20) .02 gm. 



Sodium chloride (NaCl) 0.02 gm. 



Distilled water 1,000.0 cc. 



Dissolve the peptone and salts in 100 cubic centimeters of 

 distilled water and filter. Add 400 cubic centimeters of the 

 aqueous cellulose suspension and 500 cubic centimeters of 3 

 per cent aqueous solution of washed agar (see p. 5). 



Medium 89 

 Cellulose-peptone Medium for Thermophilic Bacteria^ 



Peptone 5.0 gm. 



Calcium carbonate (CaCOs) excess 



Sodium ammonium phosphate (Na(NH4) HPO4- 



4H2O) 2.0 gm. 



Monopotassium phosphate (KH2PO4) 10 gm. 



Magnesium sulphate (MgS04-7H20) 0.3 gm. 



Calcium chloride (CaClo) 0.1 gm. 



Ferric chloride (FeCl3-6H20) trace 



Water 1,000.0 cc. 



1 ViLJOEN, J. A., E. B. Fred, and W. H. Peterson, /. Agr. Set., 16: 1, 

 1926. 



Reaction about pH 7.4. Add about 0.3 gram of paper pulp 

 or of cut up filter paper to 20 cubic centimeters of liquid in long 

 test tubes. 



