CULTURE MEDIA 41 



Reaction pH 7.1 to 7.2. 



Media 92, 93, 94 are recommended for lactic acid-forming 

 bacteria of milk and milk products. 



Medium 95 

 Malt Extract-peptone Solution 



Malt (finely ground) 30 . gm. 



Peptone 10.0 gm. 



Lactose 20 to 40 gm. 



Calcium carbonate 30 . gm. 



Tap water 1,000 . cc. 



The malt is extracted in the water by boiling for 1 hour; the 

 other constituents are then added. 



Medium 96 

 Paraffin Agar for Hydrocarbon-destroying Organisms^ 



Ammonium chloride (NH4CI) 0.5 gm. 



Dipotassium phosphate (K2HPO4) 0.5 gm. 



Magnesium sulphate (MgS04-7H20) 0.5 gm. 



Paraffin oil 10.0 gm. 



Agar 20 . gm. 



Distilled water 1,000.0 cc. 



1 Sohngen, N. L., Centr. Bakt, II Abt., 37: 595-609, 1913. 



Medium 97 

 Solution for Fat Decomposition^ 



Dipotassium phosphate (K2HPO4) 5.0 gm. 



Ammonium phosphate (NH4)3P04) 5.0 gm. 



Magnesium sulphate (MgS04-7HoO) 1.0 gm. 



Calcium chloride (CaCl2) 1.0 gm. 



Ferric chloride (FeCl3-6H20) trace 



Sodium chloride (NaCl) trace 



Distilled water 1,000.0 cc. 



2 Rahn, 0., Centr. Bakt., II Abt., 15 : 423, 1906. 



Molten fat is placed in a flask and, on inclining the flask, the 

 fat is allowed to solidify. The above nutrient solution 

 is then added. Three-tenth to five-tenth per cent of finely 

 powdered fat may also be used with the above solution. 



