QUALITATIVE AND QUANTITATIVE ANALYSIS 83 



Sterilization by Calcium Hypochlorite.^ — 1. Add 10 grams of 

 commercial chloride of lime (titrating 28 per cent chlorine) to 

 140 cubic centimeters of water. 



2. Allow the mixture to settle for 5 or 10 minutes and decant 

 the supernatant liquid. This solution should contain about 2 per 

 cent of chlorine. 



3. For seed sterilization the solution may be diluted or used 

 full strength. The volume of the liquid should be about five 

 times that of the seed. 



4. Place the seed in a sterile test tube and cover with a 1 per 

 cent chlorine solution (original solution diluted one-half). 



5. The time required for sterilizing varies with the different 

 seeds, about 6 hours for alfalfa, 8 hours for corn, and 15 hours for 

 wheat. 



1 Wilson, J. K, Am. J. BoL, 2: 420-427. 1915. 



