142 



LABORATORY MANUAL OF MICROBIOLOGY 



Carbol fuchsin, 47 



thionin (Nicolle's), 48 

 Carbon dioxide, determination of, 

 79, 80 

 from soil, 136 

 total, 80 

 Carrot extract agar, 33 



agar for yeast, 18 

 Casein hydrolysis, 42 

 Caseinate agar, 9 

 Cellulose agar, 37 



bacteria, media for, 35 

 determination of, 69 

 decomposing bacteria, 

 isolation of, 135 

 thermophilic, 134 

 decomposition 

 aerobic, 134 

 Characteristics of root nodule bac- 

 teria, 108 

 of soil population, 92 

 Chemosynthetic bacteria, 2 

 Cleaning solution, 90 

 Clostridium pasteurianu7n, 101, 114, 



118 

 Clostridium thermocellum, 134 

 Clover sucrose agar, 15 

 Cochineal, 52 



Color change of indicators, 53 

 Congo red, method for examining 

 bacteria, 96 

 negative stain, 51 

 Corn meal medium, 43 

 Crone's medium, 45 

 Crystal violet, ammonium oxalate, 



48 

 Culture media, 1 

 Czapek's solution, 13 



D 



Davisson-Parsons method for nitro- 

 gen determination, 68 



Denitrification by pure cultures, 121 

 in soil, 122 



Denitrifying bacteria, isolation of, 

 121 

 media for, 25 



Depth, effect on number, 102 

 Detmer's solution for algae, 20 

 Diphenylamine reagent, 59 

 Direct method of measuring number 



of bacteria, 104 

 Directions for media, 3 

 Dorner capsule stain, 49 

 spore stain, 50 



of nitrogen fixing, bacteria, 119 



Embedding nodules. 111 

 Enrichment media, 1 

 Erythrosin, 49 



method of staining soil bacteria, 

 51 

 Ethyl alcohol solution, 25 

 Exercises in soil microbiology, 87 



Fat decomposition, 41 

 Ferric ammonium citrate, 43 

 Ferrous carbonate medium, 32 

 Filtration of culture media, 4 

 Flemming's stain, 112 

 Frazier's gelatin, 42 

 Fungi, culture media, 12 

 in soil, 93, 98 



Gelatin, chemical composition, 5 



general properties, 5 



liquefaction, 42 

 Gelidium corneum, 5 

 Giltay's medium, 25 

 Glucose phosphate, nitrogen-free, 1 1 

 Glycerol agar, 129 

 Gram stain, 48 



Green plants, culture media, 44 

 Gum formation, 35 



H 



Hanging-drop preparations, 95 



Hansen's solution, 16 



Hay infusion for protozoa, 19 



