62 RESEARCH IN PROTOZOOLOGY 



MASTIGOPHORA 



Bodo candatiis (Diijardin) Stein, 1878. 



Description : Hagellate, polymorphic, from spherical to elongate oval, 

 from 2.5/i to 6iL in breadth to 8/i to i8/i in length, permanent cytostome, 

 minute contractile vacuole, oval kinetoplast, two small blepharoplasts from 

 which extend two flagella, one directed anteriorly, the other nearly twice 

 as long and directed posteriorly, single vesicular nucleus with large central 

 karyosome; cyst, thin-walled, oval, 5/i to 7^ in length, single nucleus, single 

 kinetoplast, and remains of two flagella. 



Ccrcomouas longicauda Dujardin, 1841. 



Description : Hagellate, amoeboid, 2/i to 15/i in length, one anteriorly and 

 one posteriorly directed flagellum adherent to body and much shorter, two 

 blepharoplasts, a single nucleus, large central karyosome, food ingested by 

 means of pseudopodia; cysts, spherical, 4/i to 7^ in length, single nucleus. 



Coproinonas subfilis Dobell, 1908. 



Description : Hagellate, oval, 7/x to 20/i in length, permanent cytostome 

 with cytopharynx, single vesicular nucleus, large central karyosome, single 

 anterior flagellum longer than body, single blepharoplast on cytopharynx, 

 contractile vacuole discharging into reservoir ; cyst, oval or spherical, 7/x to 

 8fi in diameter, thin wall, clear contents. 



Hclkcsimastix fcccicola Woodcock and Lapage, 191 5. 



Description : flageUate, oval with anterior extremity rigid and pointed, 

 4/i to 6/x in length, single flagellum originating in anterior end but adherent 

 to body and free at posterior end, single vesicular nucleus, central karyo- 

 some, minute contractile vacuole, food ingested by pseudopodia; cyst, 

 spherical, 3^ to 3.5/i in diameter, uninucleate. 



CULTIVATION METHODS 



The coprozoic protozoa are readily cultivable. Most of them w^ill 

 live indefinitely in a stale stool diluted with distilled w^ater. 



The coprozoic amoebae grow well on the following solid medium 

 devised by Musgrave and Clegg (1904) and modified by Walker 

 (1911). 



Agar 2.50 gms. 



Sodium chloride 0.05 gm. 



Liebig's beef extract 0.05 gm. 



Normal sodium hydroxide 2.00 cc. 



Distilled water 100.00 cc. 



(Sterilize in autoclave. After sterilization reaction 

 approximately neutral.) 



Feces may be streaked on this medium in Petri dishes. Growth 

 occurs at room tem]:)erature. The dishes should be inverted to pre- 



