CHAPTER XVII 



INTESTINAL FLAGELLATES IN TISSUE CULTURE 



By 



Mary Jane Hogue 

 The University of Pennsylvania 



It has always been a question whether or not the intestinal 

 flagellates are pathogenic. Some workers believe that they give off 

 substances such as toxins and enzymes which are injurious to the 

 tissues of the host. Other workers think that they cause mechani- 

 cal injury to the cells of the tissues. It has been our hope to throw 

 some light on these problems by combining the use of cultures of 

 intestinal flagellates with tissues grown in vitro. If one can grow 

 the tissue cells of the host outside of the body and then inoculate 

 them with protozoa which are parasitic in that host one should be 

 able to observe the effect of the parasite on the host and also to 

 make a comparative study of the host and protozoan cells. This 

 method involves the culturing of the protozoa so as to have large 

 quantities of them when needed for the experiment and also the 

 growing of various tissues from different animals in media in 

 which the protozoa can live. 



During the last few years the writer has been working along 

 these lines, studying the mechanical effect of TrichomovMS on the 

 intestinal cells of chick embryos (Hogue, 1928) and making a 

 comparative study of tissue cells and protozoan cells (Hogue, 

 1922a). So far the work is in its early stages and the problems 

 open to investigators are numerous and alluring. 



The cultivation of intestinal flagellates has been carried on by 

 many investigators. Boeck (1921) cultured Chilomastix mesnili 

 very successfully in a medium containing one part of human blood 

 serum to four parts of Locke's solution. 



Emhadomonas intcstinalis (IVaskia intestinalis) was grown by 

 the writer (Hogue, 1921a) on ovomucoid, Locke-Egg, ox bile 



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