170 RESEARCH IN PROTOZOOLOGY 



In the matter of tissue penetration by Endamooha dysenteries, 

 C oiincilmania laflcuri and C. dissiinilis much work is needed both 

 on culture mammals and on man, in cases running an acute and 

 also on those running a chronic course of amoebiasis. The blood- 

 stream, the lymphatics, the spleen, lung, liver and gall bladder, and 

 capillary networks generally should be investigated, for evidences 

 of amoebic invasion, especially in cases of fatal, acute amoebiasis. 

 Appendices removed in cases of chronic appendicitis should also 

 be examined for evidences of amoebic invasion, with controls of 

 stool examination for several weeks before operation. 



The sanitary and preventive aspects of amoebic infections in 

 man and domesticated animals associated with man have intimate 

 relations to certain protozoological problems which center about 

 host-parasite relations, the viability of cysts under different con- 

 ditions in stools, and in respect to light, drying up of the stool, 

 survival in dust, survival in and transit through the digestive tract 

 of the fly and of other coprophile insects. 



The extent to which transfers of the amoebic infections of man 

 can be made into domesticated animals should be determined and 

 distinctions established between the temporary maintenance of an 

 infection, and its permanent continuance in a given new host and 

 its transfer thence to other individuals of the new host species. 

 The domesticated and other animals associated with man which 

 should be tested are the pig, cat, dog, mouse, rabbit, and the 

 domestic fowl and other barnyard birds. 



