CHAPTER XLII 



STANDARD METHODS AND REAGENTS 



By 



Justin Andrews 



The Johns Hopkins University School of Hygiene and 



PubHc Health 



METHODS 



Descriptions of the following methods, most of which are 

 standard in laboratories of parasitic protozoology, are presented in 

 as reduced form as possible. More comprehensive treatments are 

 to be found in the following books on microscopic technique to 

 which the reader is referred: Aninial Micrology (Chicago) by 

 M. F. Guyer, The Microtomist's Vade-Mecmn (Philadelphia) by 

 A. B. Lee, Pathological Technique (Philadelphia) by F. B. Mal- 

 lory and J. H. Wright, and Microscopical Technique'^ (New York) 

 edited by C. E. McClung. For methods of obtaining blood from 

 animals and of making various kinds of parenteral injections into 

 animals see Chapters H and HI, respectively, of Kolmer's In- 

 fection, Iinmimity and Biologic Therapy (Philadelphia). More 

 detailed accounts of standard and specialized techniques of pro- 

 tozoological interest are to be found in Parasitic Protozoa of Man 

 (Philadelphia) by C. F. Craig, and Vol. H of Protozoology (New 

 York) by C. M. Wenyon. 



Observation of Living Protozoa 



In General. Living protozoa may be observed either with the 

 brightfield or darkfield microscope. In the first case the specimen, 

 if it be fecal, is emulsified in a sodium chloride solution of from 

 0.4 = 0.85 per cent ; a drop of the emulsion is placed on a clean 

 slide and is covered with a clean coverslip. The thickness of the 



* See particularly Chapter VIII on Protosoological Methods by D. H. 

 Wenrich. 



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