CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Dissolve one of the added nutrients 

 in amount indicated in 1. 



(2) Tube in tall tubes. Fill within a 

 centimeter of the plug. 



Sterilization: Method not given. 



Use: To determine the constituents essen- 

 tial for the growth of purpurbacteria, 

 Rhodobacterium capsulatum and Rhodo- 

 hacillus palustris (Molisch). The cul- 

 tures were incubated in diffused light. 

 The author reported generally no growth 

 using sucrose, dextrin, glycerol, ammo- 

 nium tartrate or asparagin alone, but gen- 

 erally growth with the other materials 

 and combinations listed. 



Added nutrients and variants: The author 

 suggested the following: 



(a) Add one of the following materials: 

 sucrose 1.0% 



dextrin 1.0% 

 inulin 1.0% 

 asparagin 1.0% 

 peptone 1.0% 

 glycerol 1.0% 



peptone 1.0% + sucrose 0.5% 

 peptone 1.0% + glycerol 0.5% 

 peptone 1.0% + dextrin 0.5% 

 peptone 1.0% + asparagin 0.5% 

 peptone 1.0% + inulin 0.5% 

 asparagin 0.5% + dextrin 0.5% 

 asparagin 0.5% + inulin 0.5% 

 ammonium tartrate 1.0% 



(b) Used Moldau river water added one 

 of the following materials: 

 sucrose 1.0% or 0.5% 



dextrin 1.0% or 0.5% 



inulin 1.0% or 0.5% 



asparagin 1.0% or 0.5% 



glycerol 1.0% or 0.5% 



peptone 1.0% 



peptone 1..% + sucrose 1.0% or 0.5% 



peptone 1.0% + dextrin 1.0% or 0.5% 



peptone 1.0%, + inulin 1.0% or 0.5% 



peptone 1.0% + asparagin 1.0% or 



0.5% 

 peptone 1.0% + glycerol 1.0% or 



0.5% 

 asparagin 0.5% + dextrin 0.5% 

 asparagin 0.5% + glycerol 0.5 g. 

 dextrin 0.5% + inulin 0.5% 

 ammonium tartrate 1.0% 

 sodium citrate (neutral) 0.05% 

 ammonium citrate 0.05% 



magnesium citrate 0.05% 

 potassium citrate 0.05% 

 calcium butyrate 0.05% 

 sodium acetate 0.05% 

 iron ammonium citrate 0.05% 

 iron magnesium citrate 0.05% 

 iron ammonium citrate 0.05% + gyp- 

 sum 

 infused hay 

 infused hay + iron ammonium citrate 



0.05% 

 iron potassium citrate 0.05% 

 iron ammonium tartrate 0.05% 

 iron ammonium oxalate 0.05% 

 iron glycero phosphate 0.05% 

 iron lactate 0.05% 

 iron magnesium lactate 0.05% 

 manganese peptone 0.025% 

 manganese peptone 0.025% + 



K2HPO4 0.1% 

 manganese peptone 0.025% + 



K2HPO4 0.1% + KNO3 0.1% + 



MgS04 0.1% 

 manganese peptone 0.025% + 



K2HPO4 0.1% + KNO3 0.1% 

 manganese peptone 0.025% + 



MgS04 0.1% 

 manganese peptone 0.025% + 



KNO3 0.1% 

 manganese peptone 0.025% + 



gypsum 0.1% 

 manganese peptone 0.025% + 



K4Fe (CN)6 0.1% 

 manganese peptone 0.025% + 



citrate iron peptone 

 peptone 0.5% 



peptone + MnCOs in excess 

 peptone + FeCOa in excess 

 iron albuminate 0.05% 

 iron peptonate 0.05% 

 manganese lactate 0.05% 

 manganese phospholactate 0.05% 

 manganese salicylicate 0.05% 

 manganese fluorate 0.05% + peptone 



0.5% 

 manganese citrate 0.05% 

 manganese oxalate 0.05% 

 manganese glycerinate 0.05% 

 iron sulphide — peptone 0.05% 

 manganese acetate 0.05% 



(c) Sea water without any addition. 



(d) Moldau river water without any 



addition. 

 Reference: Molisch (1907, p. 68). 



