CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



SUBGROUP I-B 



Liquid Media in which All Constituents 

 are Inorganic 



Liquid media in which all constituents 

 are inorganic may be classified most con- 

 veniently on the basis of the type of nitro- 

 gen provided. 



Key to the sections of Subgroup I-B 



A I. Nitrogen present as free or elementary 

 nitrogen only. 



Bi. Incomplete media, so-called "basal" 

 solutions, requiring the addition of other 

 nutrients Section 1 (Med. 2-25) 



B^. Complete solutions, used as media with- 

 out additions Section 2 (Med. 26-29) 



A^. Nitrogen supplied as ammonium salts. 



Bi. Incomplete media, or basal solutions. 

 Section 3 (Med. 30-56) 



B2. Complete media. 



Ci. Media primarily for organisms oxidiz- 

 ing ammonia to nitrites for growth 

 energy Section 4 (Med. 57-72) 



C2. Media not primarily for organisms oxi- 

 dizing ammonia to nitrites for growth 

 energy Section 5 (Med. 73-85) 



A3. Nitrogen supplied as nitrites. 

 Media primarily for organisms oxidizing 

 nitrites to nitrates for growth energy. 

 Section 6 (Med. 86-95) 



A4. Nitrogen supplied as nitrates. 



Bi. Incomplete or basal solutions requiring 

 the addition of other nutrients. 



Section 7 (Med. 96-106) 



B2, Complete nutrient solutions. 



Section 8 (Med. 107-113) 



SUBGROUP I-B. SECTION 1 



Inorganic basal solutions of known 

 chemical composition, nitrogen present 

 only as atmospheric nitrogen; incomplete 

 solutions requiring the addition of other 

 nutrients. 

 Ai. Containing salts of monovalent cations 



only. 



Physiological Salt Solution. (Normal 

 saline) 2 



Molar Salt Solution 3 



A2. Containing salts of monovalent and 



other cations. 

 Bi. Salts of mono and divalent cations 



present. 



Ci. Sodium salts present. 

 Di. Calcium salts present. 

 Ashby's Basal Solution. (Hoffmann and 



Hammer) 4 



von Wahl's Basal Salt Solution 5 



Locke's Solution 6 



Winogradsky's Basal Inorganic Salt 



Solution (Heinemann) 7 



Charrin and Dissard's Basal Salt Solu- 

 tion 8 



Gerlach and Vogel's Basal Solu- 

 tion 9 



Rettger, Berman and Sturges' Basal 



Solution 10 



D2. Calcium salts not present. 



Omeliansky's Basal Salt Solution 11 



Percival's Basal Salt Solution 12 



Nawiasky's Basal Salt Solution 13 



C2. Sodium salts not present. 



Stoklasa's Basal Solution 14 



Heinze's Basal Salt Solution 15 



Sohngen's Basal Solution 16 



van Delden's Basal Gypsum Solu- 

 tion 17 



Czapek's Basal Solution 18 



Buchanan's Basal Salt Solution 19 



B2. Salts of mono, di, and trivalent cations 



present. 

 Ci. Sodium Salts present. 



Fuhrmann's Basal Solution 20 



Munter's Basal Salt Solution 21 



Meyer's Basal Salt Solution. (Per- 



otti) 22 



C2. Sodium Salts not present. 

 Dox's Inorganic Salt Solution for 



Fungi (Tanner) 23 



Gage's Basal Salt Solution 24 



Bijerinck and van Delden's Basal Salt 

 Solution 25 



2. Physiological Salt Solution (Normal 

 saline) 



Constituents : 



1. Water 1000.0 cc. 



2. NaCl (c.p.) 8.5 g. 



Preparation: (1) Dissolve 2 in 1. 

 Sterilization: As desired. 



Uses: Diluent and serological work in the 



laboratory. 

 Variants: Frost used 6.0 g. NaCl per liter 

 References: Original author not found. 

 This formula was taken from Giltner 

 (1921, p. 399). Frost (1903, p. 18). 



