CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



3. Molar Salt Solution 



Constituents : 



1. Water 1000.0 cc. 



2. NaCI (best commercial 

 grade) 60.0 g. 



Preparation: (1) Dissolve 2 in 1. 



Sterilization: As desired. 



Uses: Diluent. 



References: Giltner (1921, p. 399). 



4. Ashby's Basal Solution (Hoffmann 

 and Hammer) 



Constituents : 



1. Distilled Water 1000.0 cc. 



2. KH2PO4 0.2 g. 



3. MgS04 0.2 g. 



4. NaCl 0.2 g. 



5. CaS04 0.1 g. 



6. CaCOs 5.0 g. 



Preparation : 



(1) Dissolve the KH2PO4 in a small 

 amount of water, and add tenth nor- 

 mal NaOH to neutral point to phenol- 

 phthalein. 



(2) Dissolve 3, 4, 5 and 6 in remainder of 1 



(3) Mix (1) and (2). 



(4) Add suitable carbon source. 



(5) Distribute in 20.0 cc. lots in 150 cc. 

 Erlenmeyer flasks. 



Sterilization: Method not specified. 

 Uses: For study of the nitrogen fixing 



bacteria from soil. 

 Added Nutrients: Hoffmann and Hammer 



suggest the following list of carbon 



sources: 



Mannitol 20 g. 



Maltose 20 g. 



Sucrose 20 g. 



Galactose 20 g. 



Inulin 20 g. 



Dextrose 20 g. 



Levulose 20 g. 



Dextrin 20 g. 



RafRnose 20 g. 



Lactose 20 g. 



References: Hoffmann and Hammer (1910 



p. 128). 



5. von Wahl's Basal Salt Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 1.0 g. 



3. CaCh 0.1 g. 



4. MgS04 0.3 g. 



5. NaCl 0.1 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add one of the added nutrients, or 

 combinations of added nutrients. 



Sterilization : Not specified. 



Use: Cultivation of carrot bacillus (Bacil- 

 lus daucarum) and other organisms caus- 

 ing vegetable spoilage. 



Added nutrients : The author added one of 

 the following materials or combinations 

 of materials: 



(a) peptone 10.0 g. 



fsucrose 30.0 g. 



(b) \ asparagin 10.0 g. 



[glycerol 10.0 g. 



. , fsucrose 30.0 g. 



\asparagin 1-0 g. 



,^) [glycerol 30.0 g. 



\asparagin 1.0 or 10.0 g. 



, . /lactose 30.0 g. 



\asparagin 10.0 g. 



(sucrose 5.0 g. 

 glycerol 10.0 g. 

 ammonium 



tartrate 10.0 g. 



(sucrose 5.0 g. 

 glycerol 10.0 g. 

 KNO3 10.0 g. 



(h)(KNO. 0.5g. 



^ ^ [soda 0.5 g. 



[sucrose 5.0 g. 



(i) jglucose 5.0 g. 



[glycerol 5.0 g. 



-., fglucose 30.0 g. 



[asparagin 10.0 g. 



. . /(NH4)2S04 0.0025 g. 



^^ \Na2CO3 5.0 g. 



Reference: von Wahl (1906, p. 496). 



6. Locke's Solution 



Constituents : 



1. Water 1000.0 cc. 



2. NaCl (0.9 to 1.0%)... 9.0 to 10.0 g. 



3. KCl (0.01%) 0.1 g. 



4. CaCl2 (0.02%) 0.2 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: As desired. 



Uses: Commonly used as a diluent. Tis- 

 sue neither swells nor shrinks in this solu- 

 tion. It is also used as a basis for culture 



