CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



media, various nitrogen or carbon sources 

 being added. 

 Reference: Park, Williams and Krumwiede 

 (1924, p. 122). 



7, Winogradsky's Basal Inorganic Salt 

 Solution (Heinemann) 



Constituents : 



1. Distilled Water 1000.0 cc. 



2. MgS04 0.5 g. 



3. CaCl. 0.01 g. 



4. NaCl 2.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1 

 Sterilization : Not specified. 



Uses: Used in soil bacteriological work as 

 a base to which other materials are added. 

 Reference: Heinemann (1922, p. 37). 



8. Charrin and Dissard's Basal Salt 



Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KH2PO4 0.1 g. 



3. Na2HP04 0.1 g. 



4. CaCh 0.05 g. 



5. MgS04 0.05 g. 



6. KHCO3 0.134 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Adjustment of reaction not given. 



(3) Distribute in 50.0 cc. lots in flasks 

 and add 1.0 or 0.5 g. of one of the 

 added nutrients to each flask. 



Sterilization: Autoclave at 120°C. Time 



not specified. 

 Use: To study pigment production by 

 Bacillus pyocyaneus. The authors re- 

 ported that asparagin gave the best pig- 

 ment; peptone gave a little pigment; no 

 growth using urea. 

 Added nutrients: The authors added 0.5 or 

 1.0 g. of one of the following organic 

 materials to each 50.0 cc. lot of medium: 

 peptone glycogen 



asparagin acetic acid 



urea lactic acid 



glucose 

 Reference: Charrin and Dissard (1893, p. 

 182). 



9. Gerlach and Vogel's Basal Solution 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 0.5g. 



3. CaCOs 0.5 g. 



4. NaCl 0.5 g. 



5. FeS04 some 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and one of the car- 

 bon sources listed under added nu- 

 trients in 1. 



(2) Distribute in flat bottomed flasks. 

 Sterilization : Steam for 30 minutes on each 



of 3 successive days. 



Use: Study of nitrogen assimilation by 

 azotobacter and other bacteria from the 

 soil. More nitrogen was assimilated 

 using dextrose as a source of carbon than 

 calcium propionate. Little nitrogen was 

 assimilated if the flasks were sealed after 

 inoculation. 



Added nutrients : 



(a) The authors suggested the use of the 

 following carbon sources: 



Glucose 2.0 g. 



Calcium propionate 1.0 g. 



(b) Percival used the same basic solution 

 and added 2.0 g. glucose. 



Variants: Bonazzi added 10.0 g. of glucose, 

 designated the use of deep well water 

 added 1.0 g. CaCOs, specified the addition 

 of 0.02 to 0.5 g. FeS04-7H20, and also 

 stated that 0.23 g. KNO3 and 1.264 g, 

 Ca(N03)2 might be added if desired. 



References: Gerlach and Vogel (1902, p. 

 671), Percival (1920, p. 181), Bonazzi 

 (1921, p. 339). 



10. Rettger, Berman and Sturges' Basal 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. NaCl 5.0 g. 



3. Na2S04 2.0 g. 



4. CaCl2 1.0 g. 



5. KH2PO4 1.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add one of the added nutrients listed 

 below. 



Sterilization: Generally by filtering 

 through a Berkefeld filter, in some cases 

 by heat. 



Use : To study proteolysis by Proteus vul- 

 garis, B. prodigiosus and B. subtilis. 



Added nutrients : 



(a) Various chemically pure proteins 

 added alone or in combination. 



