6 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(b) Egg albumin prepared by method of 

 Hopkins and Pinkus (1899); amount 

 not specified. 



(c) Peptone 10.0 g. 



Reference: Rettger, Berman and Sturges 

 (1916, pp. 15-33). 



11. Omeliansky's Basal Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 0.5 g. 



3. MgS04 0.03 g. 



4. NaCl 0.5 g. 



5. Na.CO, 1.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add one of the added nutrients as 

 indicated under added nutrients. 



Sterilization: The various media under 

 'added nutrients' are sterilized differently. 

 The method for each medium will be given 

 with the nutrient. 



Use: To study nitrate and nitrite produc- 

 tion from organic nitrogen. Author re- 

 ported that in the concentrations used 

 all tests for NH3, nitrites or nitrates were 

 negative. Nessler's reagent was used to 

 determine ammonia amyl iodine reaction 

 for nitrite and diphenylamine for nitrate. 



Added nutrients: The author added one of 

 the following materials to the basic solu- 

 tion: 



(a) Asparagin 1.0%. Filter through a 

 Chamberland filter to sterilize. 



(b) Urea 1.0%. Filter through a Cham- 

 berland filter to sterilize. 



(c) Bouillon 50.0 cc. Both basic solution 

 and bouillon to be sterile. Method 

 of sterilization not given. 



(d) Egg white 1.0%. Sterilize in the 

 steamer. 



(e) Fresh urine 50.0 cc. Filter through a 

 Chamberland filter to sterilize. The 

 urine gave a slight ammonia test with 

 Nessler's reagent. To remove all 

 ammonia add 1.0% soda to the fresh 

 urine, filter and store the filtrate for 

 2daysat25°C. overHsSOi. Then fil- 

 ter through a Chamberland filter as 

 above to sterilize. 



Reference: Omeliansky (1899 p. 481). 



12. Percival's Basal Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. K2HPO4 5.0 g. 



3. MgS04 2.5 g. 



4. NaCl 2.5 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add 10.0 g. of one of the added nu- 

 trients. 



Sterilization : Not specified. 



Use: To demonstrate ammonia production 



from protein. 

 Added nutrients : 



(a) Percival added 1.0% of fibrin or 

 gelatin. 



(b) Harvey added 10.0 g. of one of the 

 following materials: peptone, fibrin, 

 gelatin. 



(c) Omeliansky used 1.0 g. potassium 

 phosphate, 0.5 g. MgSO., 0.1 g. NaCl 

 in the basic solution and added one 

 of the listed proteins. He studied 

 the production of aroma by Bad. 

 ester oar omaticum. Growth with 

 aroma production took place with all 

 materials except casein and keratin. 

 One of the following materials was 

 added: 



egg albumin (Kahlbaum) 10.0 g. 



blood albumin (Kahlbaum) . . . 10.0 g. 



peptone (Okuney) 10.0 g. 



casein (Hammorsten) (Merck). 10.0 g. 



keratin (Zyzkia, Merck) 10.0 g. 



References: Percival (1920, p. 112) Harvey 

 (1921-22, p. 102), Omeliansky (1923, pp. 

 409, 411). 



13. Nawiasky's Basal Salt Solution 



Constituents : 



1. Water 1000.0 cc. 



2. NaCl 5.0 g. 



3. K2HPO4 2.0 g. 



4. MgS04 0.5 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Neutralize and make slightly alkaline 



(3) Add one of the organic materials 

 listed in added nutrients to (2). 



Sterilization: Method not given. 

 Use: Cultivation of Proteus vulgaris. 



