CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Added nutrients: 



asparagin 5.0 g 



glycocoll 4.0 g 



alanin 6.25 g 



isobutylaldehyde 1.6 g 



leucine 2.0 g 



aspartic acid 4.5 g 



Reference: Nawiasky (1908, p. 215). 



14. Stoklasa's Basal Solution 

 Constituents : 



1. Water (River, Tap) 1000.0 cc. 



2. Dipotassium phosphate 0.5 g. 



3. CaCOs 1.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add suitable carbon source. 



(3) Distribute in large Erlenmeyer flasks 

 (40 cm. X 15 cm.) 



Sterilization: Sterilize in streaming steam 



or in the autoclave. 

 Uses: To study nitrogen assimilation by 

 Azotobacter and Radiobacter as in- 

 fluenced by various carbohydrates. 

 Added nutrients: The following carbohy- 

 drates were suggested by the author to 

 serve as carbon sources: 



l-arabinose saccharose 



1-xylose rhamnose 



d-glucose lactose 



d-galactose maltose 



d-fructose 

 Reference: Stoklasa (1908 p. 491). 



15. Heinze's Basal Solution 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. CaCh 0.2 g. 



3. MgS04 0.4 g. 



4. KH2PO4 2.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add 1.0% of one of the listed added 

 nutrients 



(3) Adjustment of reaction not specified. 

 Sterilization : Not specified. 



Use : To study oxalic acid formation. Oxa- 

 lic acid was formed in quite small amounts. 

 Heavy formation of HNO3 and ammonia 

 occurred. 

 Added nutrients: The author employed 

 1.0% of one of the following: 

 peptone 

 hemialbumose 

 gelatin 

 Reference: Heinze (1905, p. 18). 



16. Sohngen's Basal Solution 



Constituents : 



1. Water 75.0 cc. 



2. K2HPO4 0.05 g. 



3. CaCOa 



Preparation : 



(1) Dissolve 2 in 1 and add 3. 



(2) Place in a liter Erlenmeyer flask. 



(3) Add suitable carbon source. 

 Sterilization : Method not specified. 

 Uses: To study the effect of filter paper 



(colloid) on bacterial processes, particu- 

 larly fixation of atmospheric nitrogen. 

 Added Nutrients: The author employed 

 the following: 



(a) 1.0 g. glucose + 0.1 g. CaCOs 



(b) 1.0 g. glucose + 0.1 g. CaCOs + 10.0 g. 

 filter paper cut in squares of 1 sq. cm. 



(c) 1.0 g. mannitol + 0.1 g. CaCOa 



(d) 1.0 g. mannitol + 0.1 g. CaCOj + 

 10.0 g. filter paper 



Reference: Sohngen (1913, p. 628). 



17. van Delden's Basal Gypsum Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Gypsum 0.4 g. 



3. K2HP04 0.5 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Additional nutrients are added as in- 

 dicated below. 



Sterilization: Not specified. 



Use: To study sulphate reduction by Mi- 



crospira desulfuricans. 

 Added nutrients and variants : The author 



used one of the following combinations: 



(a) Added 0.5 g. glucose and 0.5 g. pep- 

 tone. 



(b) Added 1.0 g. glucose, 1.0 g. CaCO,, 

 specified the use of tap water and used 

 1.2 g. gypsum. 



(c) Specified the use of tap water, added 

 0.5 g. NH4CI, added 0.5 g. glucose and 

 used 1.2 g. gypsum. 



(d) Specified the use of tap water, added 

 0.5 g. sodium acetate, 0.5 g. asparagin 

 and used 1.2 g. gypsum. 



(e) Specified the use of tap water, added 

 1.0 g. asparagin, used 1.2 g. gypsum 

 and added 1.25 g. of sodium succinate 

 or potassium succinate or calcium 

 citrate or potassium tartrate or po- 

 tassium malate. 



