CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Peptone (Witte) 1.0% + KNO3 0.1 or 

 1.0% 



Amygdalin 2.0% 



Amygdalin 2.0% + ammonium phos- 

 phate 0.5% 



Salicin 2.0% + ammonium phosphate 

 0.5% 



Salicin 2.0% 



Ammonium phosphate 

 References: Buchanan (1909, pp. 382, 391, 



62), Tanner (1909, p. 57) 



20. Fuhrmann's Basal Solution 



Constituents : 



1. Water 1000.0 cc. 



2. KH2PO4 1.0 g. 



3. CaCla 0.1 g. 



4. MgS04-7H20 0.3 g. 



5. NaCl 0.1 g. 



6. FesCle 0.01 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Distribute in 100.0 cc. lots. 

 Sterilization: Method not specified. 

 TJses: To study the availability of various 



nitrogen and carbon sources for Pseudo- 

 monas cerevisiae. 

 Added Nutrients : Make the following addi- 

 tions to each 100.0 cc: 



(a) Asparagin 1.0 g. 



(b) Asparagin 1.0 g. + glucose 1.5 g. 



(c) Asparagin 1.0 g. + sucrose 0.75 g. 



(d) Potassium nitrate 1.0 g. + glycerol 

 1.0 g. 



(e) Potassium nitrate 1.0 g. + sucrose 

 0.75 g. 



(f) Potassium nitrate 1.0 g. + glucose 

 1-5 g. 



(g) Ammonium chloride 1.0 g. + sucrose 

 0.75 g. 



(h) Ammonium chloride 1.0 g. + glucose 

 1.5 g. 

 Reference: Fuhrmann (1906, p. 319). 



21. Miinter's Basal Salt Solution 



Constituents : 



1. Water 1000.0 cc. 



2. MgSOi 0.5 g. 



3. NaCl 0.5 g. 



4. CaCl2 0.1 g. 



5. K2HPO4 1.0 g. 



6. FeCls trace 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Neutralize with CaCOs and filter. 



(3) Distribute in 100.0 cc. lots in Erlen- 

 meyer flasks. 



(4) Add 0.5 g. of one of the added nu- 

 trients to each flask. 



Sterilization: Not specified. 

 Use: To study the availability of nitrogen 

 for Actinomycetes odorifer, Act. chromo- 

 genes, Act. albus I and II, Act. S. a, b, 

 and c. Good growth was obtained with 

 albumin, hemi albumin, casein, asparagin, 

 and alanine; little growth obtained with 

 tyrosin, while generally no growth with 

 urea, sulphocarbamide, or dicyandiamide. 

 Added nutrients: The author employed 0.5 

 g. of one of the following nitrogen sources 

 for each 100.0 cc. of medium: 

 albumin urea 



hemi albumin sulphocarbamide 



casein alanine 



asparagin tyrosine 



dicyandiamide 

 Reference: Munter (1913 p. 373). 



22. Meyer's Basal Salt Solution (Perotti) 



Constituents : 



1. Distilled water 1000.0 cc. 



2. K2HPO4 10 g. 



3. CaCh 0.1 g. 



4. MgS04 0.1 g. 



5. xNaCl 0.1 g. 



6. Iron chloride 0.01 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Uses: Used by Perotti as a basis for the 



preparation of various solutions for the 



study of the dicyandiamid bacteria of 



the soil. 



Variants : 



(a) Buchanan used a basal solution con- 

 taining the following materials: 



1. Water 1000.0 cc. 



2. KH0PO4 0.1 g. 



3. CaCl. 0.1 g. 



4. MgS04-7H20 0.3 g. 



5. NaCl 0.1 g. 



6. Fe2Cla 0.01 g. 



Buchanan added one of the following 

 materials, and used the media to deter- 

 mine the constituents essential for the 

 growth of Bacillus thermophilus. Growth 

 was obtained with all materials except as- 

 paragin alone. 



asparagin 1.0% 



asparagin 1.0% + glycerol 1.0% 



