10 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



asparagin 1.0% + filter paper 1.0% 

 urea 1.0% + peptone 1.0% 

 peptone 1.0% + glucose 

 peptone 1.0% + lactose 1.0% 

 peptone 1.0% + sucrose 1.0% 



(b) Klaeser added 2.0g KNO3 and lO.Og. 

 of glucose. He used the solution to 

 study nitrate reduction. 



(c) Stapp used l.Og HK2PO4 instead 

 of KH2PO4. He dissolved 3.0g 

 Na2HP04 and 0.5 g uric acid in 45.0 

 cc. water and added 50.0 cc. of the basal 

 solution. The medium was used for 

 the isolation of uric acid splitting 

 bacteria from the feces and soil. The 

 organisms studied were Bad. co- 

 bay ae, Bad. capri, Bad. guano, Bad. 

 muscili, Bad. hoUandicus. 



Reference : Buchanan (1906 p. 73). Perotti 

 (1908 p. 220), Klaeser (1914 p. 38a) Stapp 

 (1920 p. 3). 



23. Box's Inorganic Salt Solution For Fungi 

 (Tanner) 



Constituents : 



1. Distilled Water 3000.0 cc. 



2. MgS04 1.5 g. 



3. K2HPO4 3.0 g. 



4. KCl 1.5 g. 



5. FeS04 0.03 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization : Not specified. 



Uses: Cultivation of fungi. 

 Reference: Tanner (1919 p. 65). 



24. Gage's Basal Salt Solution 



Constituents : 



1. Water 1000.0 cc. 



2. KH2PO4 0.5 g. 



3. MgS04 0.2 g. 



4. CaCl2 0.02 g. 



5. Fe2(S04)3 1 drop of 10% solution. 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add 1.5 g. of suitable carbon source. 

 Sterilization: Method not specified. 

 Uses : Study of nitrogen fixation and of the 



morphology of the nitrogen fixing bacteria. 

 Added nutrients: The author suggests the 

 addition of 1.5 g. of one of the following 

 carbon sources: 



Mannitol Galacton from slip- 



Glucose pery elm. 



Dulcitol Pentosan from dulse 



Sorbitol Sinistrin 



Sucrose Lichenin 



Galactose 

 Maltose 



Mannan from Salep 

 Reference: Gage (1910 p. 21). 



25. Beijerinck and van Delden's Basal 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. MgS04.7H20 8.0 mg. 



3. MnS04.4H20 0.5 mg. 



4. FeCl3.3H20 0.5 mg. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Uses: Used by Beijerinck and van Del- 

 den as a mineral mixture to be added in 

 small quantities to a considerable variety 

 of liquid media. 

 Reference: Beijerinck and van Delden 

 (1903 p. 41). 



SUBGROUP I-B. SECTION 2 



Inorganic liquid media of known composi- 

 tion, nitrogen as free nitrogen only, com- 

 plete solutions, used as media without 

 addition of other nutrients. 



"Complete" inorganic non-nitrogenous 

 media have been described for the cultiva- 

 tion of certain iron bacteria and for certain 

 algae. In each case it will be noted that 

 either "well water" or a heavy inoculum of 

 soil or soil extract is used. It is probable 

 therefore that ammoniacal or nitrate nitro- 

 gen is usually present, and may in some 

 cases be essential for the growth of the mi- 

 croorganisms. 



The important media of this section may 

 be differentiated as follows: 

 Ai. Medium employed primarily for iron 



bacteria. 



Ellis' Ferric Hydroxide Solution 26 



A2. Media for algae. (Cyanophyceae or 



blue-green). 



Beijerinck's Basal Phosphate Solution. 27 



Heinze's Basal Solution A 28 



Heinze's Basal Solution B 29 



26. Ellis' Ferric Hydroxide Solution 

 Constituents: 



1. Well water. 



2. Ferric hydroxide. 



