CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



11 



Preparation: (1) Add freshly precipitated 

 ferric hydroxide (amount not specified) 

 to sterile well water in sterile flask. 



Uses: Inoculate with Spirophyllum fer- 

 rugineum (one of the iron bacteria) and 

 expose to sunlight. 



Reference: Ellis (1907 p. 511). 



27. Beijerinck's Basal Phosphate Solution 



Constituents : 



1. Water 100.0 cc. 



2. K2HPO4 0.02 g. 



Preparation : 



(1) Dissolve 2 in 1. 



(2) Place in a 3 liter flask and plug with 

 cotton. 



Sterilization : Not specified. For purposes 

 used, probably generally unnecessary. 



Uses: Add an inoculum of 1.0 or 2.0 g. 

 garden earth and incubate at 16°-20°C. 

 (In the light?) Oligonitrophilic organ- 

 isms of the Cyanophyceae (blue-green 

 algae) will develop, the medium becom- 

 ing bluish green. 



Variants: van Delden specified the use of 

 Grabenwasser." 



Reference: Beijerinck (1901 p. 562), van 

 Delden (1903-04 p. 85). Lohnis (1913 

 p. 115). 



28. Heinze's Basal Solution A 



Constituents : 



1. Distilled water 1000.0 cc. 



2. KHsPOi 2.0 g. 



3. CaCla 0.2 g. 



4. FeCls in 10% solution... 20 drops. 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. If K3PO4 is 

 used, the solution may be acidified 

 with sulphuric acid and neutralized 

 with soda. 



(2) Distribute in 100 cc. lots. 

 Sterilization: Not specified. 



Uses : Inoculate with 1.0 cc. of a suspension 

 of 1.0 g. of soil in 100.0 g. sterile distilled 

 water. Incubate (in the light?). The 

 soil Cyanophyceae (blue green algae) will 

 develop. Nitrogen may possibly be 

 assimilated (fixed) in small amounts. 



Variants: The author substituted K2HPO4 

 or K3PO4 for KH2PO4. 



Reference: Heinze (1906 p. 703). 



29. Heinze's Basal Solution B 



Constituents : 



1. Water 1000.0 cc. 



2. Potassium phosphate neu- 



tral 0.2 g. 



3. MgS04 0.2 g. 



4. K2SO4 0.2 g. 



5. CaCOa 0.1 g. 



6. Iron chloride trace 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 



Sterilization: Not specified. 



Uses: Inoculate with a suspension of soil. 

 Soil algae, such as Nostoc punctiforme 

 will develop. If soil bacteria are present 

 Nostoc punctiforme will grow and nitro- 

 gen will be assimilated. 



Reference: Heinze (1906 p. 653). 



SUBGROUP I-B. SECTION 3 



Inorganic liquid solutions of known com- 

 position; nitrogen supplied as ammonium 

 salts; incomplete or basal solutions re- 

 quiring the addition of other nutrients. 



The several solutions falling in this sec- 

 tion may be differentiated as follows: 

 Ai.* Containing ammonia as ammonium 

 chloride. 



Boas' Basal Ammonium Chloride Solu- 

 tion 30 



Wherry's Basal Ammonium Chloride 



Solution B 31 



Kendall, Walker and Day's Basal Am- 

 monium Chloride Solution 32 



Lohnis' Basal Ammonium Chloride 



Salt Solution 33 



A2. Containing ammonia as ammonium 



sulphate. 

 Bi. Not containing additional salts. 

 Buchanan's Basal Ammonium Sul- 

 phate Solution 34 



Cathelineau's Basal Ammonium Sul- 

 phate Solution 35 



B2. Containing additional salts. 

 Pere's Basal Ammonium Sulphate 



Solution 36 



Henneberg's Basal Ammonium Sul- 

 phate Solution 37 



Omeliansky's Basal Ammonium Sul- 

 phate Solution 38 



See A2, A3, A4 and A5 



