12 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Kita's Basal Ammonium Sulphate 

 Solution 39 



Grimm's Basal Ammonium Sulphate 

 Solution 40 



Waksman and Joffe's Basal Sulphur 

 Ammonium Sulphate Solution 41 



Bokorny's Basal Ammonium Sulphate 

 Solution, (Vierling) 42 



Verkade and Sohngen's Basal Ammo- 

 nium Sulphate Solution 43 



Starkey's Basal Ammonium Sulphate 



Solution 44 



A3. Containing ammonia as salts of phos- 

 phoric acid. 



Ayers and Rupp's Basal Sodium Am- 

 monium Phosphate Solution 45 



Committee S. A. B. Basal Ammonium 

 Phosphate Solution 46 



Kendall, Day and Walker's Basal Am- 

 monium Phosphate Solution 47 



Laurent's Basal Ammoniima Phosphate 

 Solution 48 



Fermi's Basal Ammonium Phosphate 

 Solution 49 



Palladin's Basal Ammonium Phos- 

 phate Solution 50 



Schukow's Basal Ammonium Phos- 

 phate Solution 51 



Koser's Basal Ammonium Phosphate 



Solution 52 



A4 Containing ammonia as ammonium 



nitrate. 



Munter's Basal Ammonium Nitrate 

 Solution 53 



von Bronsart's Basal Ammonium Ni- 

 trate Solution 54 



Adolf Mayer's Basal Ammonium Ni- 

 trate Solution (Smith) 55 



As. Containing ammonia as Ammonium 



Carbonate. 



Prazmowski's Basal Ammonium Car- 

 bonate Solution (Smith) 56 



30. Boas' Basal Ammonitun Chloride 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. NH4CI (0.25%) 2.5 g. 



3. KH2PO4 (0.25%) 2.5 g. 



4. MgS04 (0.15%) 1.5 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add 5.0% of one of the carbohydrates. 



(3) Distribute in 40.0 cc. lots in 100.0 cc, 

 Erlenmeyer jena glass flasks. 

 Sterilization: Not specified. 

 Uses : Used to study the formation of starch 

 by molds, Aspergillus orijzae. If starch 

 is formed, a blue color appears when iodine 

 is added to the culture. 

 Added nutrients: The author used 5.0% 

 of one of the following carbon sources: 

 sucrose glucose 



levulose galactose 



maltose 

 Reference: Boas (1922 p. 8). 



31. Wherry's Basal Ammonium Chloride 

 Solution B 



Constituents: 



1. Redistilled water 1000.0 cc. 



2. NajCOa 1.0 g. 



3. KCl 1.0 g. 



4. CaCl2 1.0 g. 



5. MgS04 1.0 g. 



6. NH4CI 2.0 g. 



7. KH2PO4 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, and 4 in 1. This is 

 called solution "B". 



(2) Dissolve 6 and 7 in (1). 



(3) Distribute in 50 cc. lots and add one 

 of the test materials to each lot. 



Sterilization: Not specified. 



Use: To study acid fastness of tubercle 

 bacilli. Growth started first on methyl 

 alcohol. After 20 days the order of vigor 

 of growth was as follows: Propyl, butyl, 

 ethyl and methyl alcohol. Culture be- 

 came strongly acid fast only in propyl 

 alcohol. 



Added nutrients: The following carbon 

 sources were used: 



ethyl alcohol 2.0% 



propyl alcohol 2.0% 



amyl alcohol 2.0% 



butyl alcohol 2.0% 



methyl alcohol 2.0% 



leucine 0.1 g. 



tyrosine 0.1 g. 



alanine 0. 1 g. 



glycocoU 0.1 g. 



aspartic acid 0.1 g. 



glutamic acid 0.1 g. 



(Salts to be Kahlbaum C. P.) 



Reference: Wherry (1913 p. 116). 



