CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



13 



32. Kendall, Walker and Day's Basal Am- 

 monium Chloride Solution 



Constituents: 



1. Redistilled water 1000.0 cc. 



2. NH4CI 4.0 g. 



3. NaCl 5.0 g. 



4. Na2HP04 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the nutrients listed below. 



(3) Distribute in 100.0 cc. quantities in 

 flasks. 



Sterilization: Method not given. 



Use: To study lipase production by tuber- 

 cle bacilli. Different esters were added 

 to clear bacteria free culture broth and 

 incubated for 24 hours. Amount of acid 

 produced measured in terms of N/50 

 NaOH, determined lipase production. 



Added nutrients: The authors used ethyl 

 alcohol, glycerol or mannitol as carbon 

 sources. 



Reference: Kendall, Walker and Day (1914 

 p. 455). 



33. Lohnis' Basal Ammonium Chloride 



Solution 

 Constituents: 



1. Water (tap) 1000.0 cc. 



2. NH4CI 0.5 g. 



3. K2HPO4 0.5 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add 1.0% of one of the listed nutrients 

 Sterilization: Not specified. 



Use: Methane production by cellulose de- 

 composers. 

 Added nutrients: The author added 1.0% 

 of one of the following materials: 

 acetate starch 



butyrate sugar 



lactate peptone 



gum wool 



Reference: Lohnis (1913 p. 93). 



34. Buchanan's Basal Ammonium Sulphate 



Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. (NH4)2S04 (0.5%) 5.0 g. 



Preparation : 



(1) Add 2.0% of one of the carbon sources 

 to water and dissolve. 



(2) Then add 0.5% ammonium sulphate 

 to (1). 

 Sterilization: Sterilize on each of 3 succes- 

 sive days for 20 minutes in flowing steam. 

 Use : To study gum production of Bacillus 



radicicola. 

 Added nutrients: The following carbon 

 sources (2.0%) were used by the author: 

 arabinose levulose lactose 



rhamnose melitose raffinose 



glucose inositol mannitol 



mannose sucrose inulin 



galactose maltose starch 



Reference: Buchanan (1909 p. 388). 



35. Cathelineau's Basal Ammonium Sul- 

 phate Solution 



Constituents : 



1. Water 100.0 cc. 



2. (NH4)2S04 1.0 g. 



Preparation : 



(1) Dissolve 2 in 1. 



(2) Add one of the following to (1). 



(a) Glucose 5.0 g. 



(b) Sodium succinate 5.0 g. 



(c) Sodium phosphate 5.0 g. 



(d) Sodium succinate 5.0 g. 



Glucose 5.0 g. 



(e) Glucose 5.0 g. 



Sodium phosphate 5.0 g. 



(f) Sodium succinate 5.0 g. 



Sodium phosphate 5.0 g. 



Sterilization: Method not given. 



Use: To study pigment production and 



fluorescence by Bacillus viridis (Lesage). 



Pigment production was independent of 



the amount of phosphates present. 

 Added nutrients: The author added the 



following carbon sources and salts: 



(a) Glucose 5.0 g. 



(b) Sodium succinate 5.0 g. 



(c) Glucose 5.0 g. 



Sodium succinate 5.0 g. 



(d) Glucose 5.0 g. 



Sodium phosphate 5.0 g. 



(e) Sodium succinate 5.0 g. 



Sodium phosphate 5.0 g. 



Reference: Cathelineau (1896 p. 235). 



36. Pere's Basal Ammonium Sulphate 

 Solution 



Constituents : 



1. Water 100.0 cc. 



2. Ammonium phosphate 1.0 g. 



