14 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



3. (NH4)2S04 0.5 g. 



4. Potassium phosphate 0.25 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the test materials in 

 amounts given to (1). 



(3) The reaction must be neutral. If 

 acid after sterilization, add a few- 

 drops of ammonia. 



Sterilization: Method not given. 



Use: To study the decomposition of car- 

 bonaceous bodies (polyatomic alcohols, 

 starch, etc.) by Tyrothrix tenuis, Bacilhis 

 mesentericus vulgatus and Bacillus sub- 

 tilis. 



Added nutrients: The author used the 

 following substances: 



Mannitol 10.0 g. 



Glycerol 5.0 g. 



Glycerose, amount not given 



Potato starch 2.0 g. 



Sucrose 5.0 g. 



Glucose 5.0% = 5.0 g 



Reference: Per6 (1896 p. 421). 



37. Henneberg's Basal Ammonium Sul- 

 phate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KH2PO4 3.0 g. 



3. (NH4)2S04 3.0 g. 



4. MgS04 2.0 g. 



5. Carbon source. 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Do not adjust the reaction. 



(3) Add any desired carbon source. 

 Sterilization: Not specified. 



Use : To study a suitable carbon source for 

 acetic acid organisms, B. pasteurianum, 

 B. aceti, B. oxydans and B. Kutzingianum. 

 Glucose was found to be a suitable carbon 

 source, but methyl, ethyl or propyl alco- 

 hol, acetic acid or ammonium tartrate 

 were not suitable sources of carbon. 



Reference: Henneberg (1898 p. 18). 



38. Omeliansky's Basal Ammonium Sul- 

 phate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 0.1 g. 



3. (NH4)2S04 0.1 g. 



4. MgS04 0.05 g. 



5. NaCl Trace 



6. Chalk 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Prepare a 1.0% solution of one of the 

 test materials. 



(3) Distribute in test tubes. 



(4) Add chalk to each tube. 

 Sterilization: Not specified. 



Use: To study the fermentation ability of 

 Bacterium for micicum under either anaero- 

 bic or aerobic conditions. 

 Added nutrients : The author used 10 grams 

 of the following carbon sources: 

 Glucose Dextrin 



Sucrose Amylum 



Galactose Inulin 



Lactose Arabinose 



Mannitol Gum arable 



Dulcitol Ethylene glycol 



Maltose Glycerol 



Erythritol 

 Reference: Omeliansky (1903-4 p. 258). 



39. Kita's Basal Ammonium Sulphate 

 Solution 



Constituents : 



1. Water 1000.0 cc. 



2. MgS04 0.25 g. 



3. KH2PO4 5.0 g. 



4. FeCla solution drops 



5. (NH4)2S04 5.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 5 in 1. 



(2) Add several drops of a ferric chloride 

 solution to (1). 



(3) Add 50.0 g. of any desired carbohy- 

 drate to (2). 



Sterilization: Not specified. 



Use: To study utilization of various carbon 

 sources by molds, Aspergillus okazaki, 

 Aspergillus candidus, Aspergillus albus, 

 Aspergillis lamarii, Pseudorhizopus, As- 

 pergillus glaucus. 



Reference: Kita (1913 p. 434). 



40. Grimm's Basal Ammonium Sulphate 

 Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. K2HPO4 0.5 g. 



3. MgS04 0.5 g. 



4. (NH4)2S04 5.0 g. 



