CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



15 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Distribute in 30.0 cc. lots in 200.0 cc. 

 Erlenmeyer flasks. 



(3) After sterilization add one of the test 

 materials to (2). (Amount not speci- 

 fied). 



Sterilization: Not specified. 



Use: To study the utilization of various 



carbon sources by Oospora lactis and 



Aspergillus repens. Growth with ethyl 



alcohol, isobutyl alcohol, acetic acid, 



ethyl ether, propyl, isopropyl and amyl 



formic acid, methyl, ethyl, propyl, iso- 

 propyl, isobutyl and amyl acetic acid. 



Other materials give very little or no 



growth. 

 Added nutrients: The author used the 



following carbon sources: 



Pentan 



Heptan 



Hexan 



Octan 



Methyl alcohol 



Ethyl alcohol 



Propyl alcohol 



Isopropyl alcohol 



Butyl alcohol 



Isobutyl alcohol 



Amyl alcohol 



Formic acid 



Acetic acid 



Propionic acid 



Butyric acid normal 



Isobutyric acid 



Valeric acid 



Ethyl ether 



Propyl ether 



Formaldehyde 



Acetaldehyde 



Propionic aldehyde 



Methyl formic acid 



Ethyl formic acid 



Propyl formic acid 



Isobutyl formic acid 



Methyl acetic acid 



Ethyl acetic acid 



Propyl acetic acid 



Isobutyl acetic acid 



Methyl iodine 



Ethyl iodine 



Ethyl bromide 



Propyl bromide 



Chloroform 

 Reference: Grimm (1914 p. 648). 



41. Waksman and Joffe's Basal Sulphur 



Ammonium Sulphate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. (NH4)2S04 2.0 g. 



3. K2HP0< 1.0 g. 



4. MgS04 0.5 g. 



5. KCl 0.5 g. 



6. FeS04 0.01 g. 



7. Sulfur 10.0 g. 



8. Ca3(P04)2, 2.5 or 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7, 8 and one of 

 the added nutrients in 1. 



(2) Distribute in 100.0 cc. portions in 

 250.0 cc. flasks. 



Sterilization : Sterilize in flowing steam for 

 30 minutes on 3 consecutive days. 



Use: Enrichment and isolation of sulphur 

 bacteria, Thiobacillus thiooxidans. If 

 glucose be omitted fungi do not grow. 

 Medium supports the growth of all sul- 

 phur oxidizers. 



Added nutrients: 



(a) The authors added 10.0 glucose or any 

 other organic or inorganic stimulator. 



(b) Used the basic solution without any 

 additions. 



Reference: Waksman and Joffe (1922 

 p. 239). 



42. Bokomy's (Vierling) Basal Ammonium 



Sulphate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. (NH4)2S04 1-0 g. 



3. KH2PO4 0.2 g. 



4. MgS04 0.1 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add 2.0 g. of one of the carbon sources 

 Sterilization: Not specified. 



Use: Author used the solution to study 

 urease production. 



Added nutrients: The author added the 

 following carbon sources: 

 Glycerol Arabinose 



Ethyl alcohol Sorbose 



Lactose Xylose 



Galactose Mannose 



Rhamnose 



Reference: Vierling (1920 p. 31). 



