16 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



43. Verkade and Sohngen's Basal Ammo- 

 nium Sulphate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. K2HPO4 (0.05%) 0.5 g 



3. MgS04 (0.05%) 0.5 g 



4. FeCU (0.01%) 0.1 g 



5. MnS04 (0.01%) 0.1 g 



6. (NH4) 2SO4 (0.05%) 0.5 g 



7. CaCOa 

 Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add 0.25% of one of the test materials 

 and an excess of CaCOs. 



Sterilization: Autoclave at 110°C. for 10 



minutes. 

 Use: To study the availability of organic 



acids as a carbon source for Aspergillus 



niger and Pcnicillium glaucum. Fumaric, 



citric aconitic, oleic and erucic acids 



give good growth. 

 Added nutrients: 



maleic acid 



fumaric acid 



citric acid 



itaconic acid 



phenylitaconic acid 



allocinnamic acid 



cinnamic acid 



aconite acid 



isocrotonic acid 



crotonic acid 



BB-dimethylacrylic acid 



angelic acid 



tiglic acid 



oleic acid 



elaidic acid 



erucic acid 



brassidic acid 

 Variants: The authors substituted 0.05% 



KNO3 for (NH 4)280 4. 

 Reference: Verkade and Sohngen (1920 



p. 82). 



44. Starkey's Basal Ammonium Sulphate 

 Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. (NH4)2S04 0.2 to 0.4 g. 



3. KH2PO4 3.0 to 4.0 g. 



4. CaCls 0.25 g. 



5. MgS04 0.5 g. 



6. FeSOi 0.01 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add one of the added nutrients. 

 Sterilization : Heat in flowing steam for 30 



minutes on each of three consecutive 

 days. 

 Added nutrients : The author added one of 

 the following: 



(a) Sulphur 1.0%. The sulphur was 

 weighed out and placed in individual 

 flasks, the basic solution added and 

 sterilized. 



(b) Sodium thiosulphate 0.5%. The 

 thiosulphate was dissolved in water, 

 sterilized and added to the sterile 

 basic solution in the necessary 

 amounts. 



Use: Cultivation of Thiobacillus thioxidans 



and to study oxidation of sulphur. 

 Reference: Starkey (1925 p. 138). 



45. Ayers and Rupp's Basal Sodium Ammo- 

 nium Phosphate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Sodium ammonium phosphate. . .1.5 g. 

 Preparation : 



(1) Dissolve 2 in 1. 



(2) Add one of the combinations given 

 under added nutrients. 



(3) Adjust to pH = 7.0 

 Sterilization: Not specified. 



Use: To study acid and alkali production. 



Added nutrients and modifications: The 

 author added one of the following com- 

 binations: 



(a) Glucose 5.0 g. 



citric acid (neutralized with 



NaOH) 2.5 g. 



KCl 0.2 g. 



(b) glucose 5.0 g. 



KCl 0.2 g. 



(c) citric acid (neutralized with 



NaOH) 2.5 g. 



KCl 0.2 g. 



(d) Used 3.6 g. sodium ammonium phos- 

 phate in the basic solution and added 

 KH2PO4 1.2 g. and glucose 5.0 g. To 

 this solution was added 1.2 g. of one 

 of the following acids neutralized 

 with NaOH: formic acid, acetic acid, 

 lactic acid, succinic acid. 



(e) Same as (d) without the addition of 

 acids. 



