CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



17 



Reference: Ayers and Rupp (1918 pp. 199, 

 209). 



46. Committee S. A. B. Basal Ammonium 

 Phosphate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. (NH4)H2P04 1.0 g. 



3. KCl 0.2 g. 



4. Brom Cresol Purple (Satu- 

 rated aqueous solution) 2.0 cc. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Adjust to pH = 7 by addition of 

 NaOH. 



(3) Add fermentable substances. 



(4) Tube. 



Sterilization: Method not specified. 



Uses : To test the ability of microorganisms 

 to produce acid in a solution having 

 ammonia as the nitrogen source and a 

 suitable fermentable carbon compound. 



Added nutrients: Suitable amounts of any 

 of the carbohydrates or of the polyatomic 

 alcohols, etc., may be used. 



Variant. Authors also prepared the me- 

 dium as follows: 



(1) Dissolve 1.0 g. (NH4)H2P04, 0.2 g. 

 NaCl, 0.2g.MgSO4 and 10.0 g. of one 

 of the added nutrients in 1000.0 cc. 

 water. 



(2) Adjust to pH = 7.0 by the addition of 

 NaOH. 



(3) Indicator may be added: 

 Litmus 



Phenol red, 2.0 cc. saturated aqueous 



solution per liter 

 Brom cresol purple, 2.0 cc. saturated 



aqueous solution per liter 

 Brom cresol purple, 1.0 cc. saturated 



aqueous solution per liter 

 Cresol red, 1.0 cc. saturated aqueous 



solution per liter 

 Brom cresol green 2.0 cc. of a 2.0% 



alcoholic solution per liter 

 Brom-chlor phenol blue 5.0 cc. of 



saturated aqueous solution per liter 



Reference : Committee Society of American 



Bacteriologists (1922 p. 523), (1923 p. 11). 



47. Kendall, Day and Walker's Basal 

 Ammonium Phosphate Solution 



Constituents: 



1. Redistilled water 1000.0 cc. 



2. (xNH4)2HP04 4.0 g. 



3. NaCl 5.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add one of the added nutrients listed 

 below. 



(3) Distribute in 100.0 cc. lots. 

 Sterilization: Not specified. 



Use: To study metabolism and lipase pro- 

 duction by tubercle bacilli. Authors 

 used alizarin, neutral red and phenol- 

 phthalein to study reaction changes. 

 Ziehl-Nielsen stain for staining. Differ- 

 ent esters were added to clear bacteria 

 free culture broth, incubated for 24 hours 

 and the amount of acid produced, (meas- 

 ured in terms of N/50 NaOH) determined 

 lipase production. 



Added nutrients: The authors added one 

 of the following nutrients: 



mannitol 10.0 g. 



glycerol 30.0 g. 



sodium acetate 



Reference: Kendall, Day and Walker (1914 

 p. 434), Kendall, Walker and Day (1914 

 p. 455). 



48. Laurent's Basal Ammonium Sulphate 

 Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Ammonium phosphate (neu- 

 tral) 2.5 g. 



3. Potassium phosphate (neu- 

 tral) 2.5 g. 



4. MgS04 1.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: As desired; not specified. 

 Uses : Used with the addition of 1.0% of any 



one of a considerable variety of nitroge- 

 nous and carbon compounds. 

 Added nutrients : 



(a) The following were employed: 

 saccharose potassium lactate 



lactose potassium citrate 



glucose potassium tartrate 



mannite ammonium bimalate 



glycerine sodium butyrate 



peptone sodium hippurate 



asparagin sodium formate 



potassium sue- potassium acetate 



cinate 

 These were used by Laurent in his 



