18 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



studies of Bacillus fluorescens 

 putidus. 

 (b) Laurent cultivated Sclerotinia Liber- 

 tiana using the basic solution and 

 adding 1.0% peptone, 2.5% lactose, 

 2.5% glycerol and 2.5% potassium 

 tartrate. 

 Variants : Smith cultivated plant parasites 

 on a medium composed of 2.0 g K2HPO4, 

 0.1 g ammonium phosphate, 0.19 MgS04, 

 and used 5.09 sodium formate or acetate 

 as an added nutrient. 

 Reference: Laurent (1899 p. 43, 81). Smith 

 (1905 p. 50). 



49. Fermi's Basal Ammonium Phosphate 



Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. Ammonium Phosphate 



(0.5 to 1.0%) 5.0 to 10.0 g. 



3. KH2PO4 (0.5%) 5.0 g. 



4. MgS04 (0.5%) 5.0 g. 



5. K3PO4 (0.05%) 0.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add one of the added nutrients. 

 Sterilization: Not specified. 



Use: Cultivation of schizomycetes. 

 Added nutrients: The author added 5.0% 



glycerol or 5.0% sucrose. 

 Variants: Tanner gave the following as 



Fermi's Solution: 



1. Distilled water 1000.0 cc. 



2. MgS04-7H20 0.2 g. 



3. K2HPO4 1.0 g. 



4. (XH4).HP04 10.0 g. 



5. Glycerol 45.0 g. 



Reference: Fermi (1892 p. 26), Tanner 



(1919 p. 68). 



50. Palladin's Basal Ammonium Phosphate 



Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. Ammonium phosphate 4.7 g. 



3. Potassium phosphate 3.0 g. 



4. MgS04 1.0 g. 



5. CaClj 1.0 g. 



6. FeCl2 Trace 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add sufficient carbohydrate to make 

 solution one fourth molar. 



(3) Distribute in thin layers in Erlen- 

 meyer flasks. 

 Sterilization : Method not specified. 

 Use: Cultivation of Chlorothecium sac- 



charophilnm. 

 Variants: The author suggests the follow- 

 ing carbon sources: 



saccharose glucose 



mannitol raffinose 



Reference: Palladia (1903 p. 146). 



51. Schukow's Basal Ammonium Phosphate 



Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. Ammonium phosphate 5.0 g. 



3. Potassium phosphate 1-0 g. 



4. MgS04 0.5 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add 9.0 to 10.0% of one of the organic 

 acids to (1). 



(3) Adjustment of reaction not specified. 



(4) Distribute into fermentation flasks 

 (sealed with H2SO4). 



(5) After inoculation plug the flask with 

 a cork and seal with paraffin. 



Sterilization: Sterilize in a steamer 

 (method not given). 



Use: To study the utilization of acids by 

 yeast. The author found 0.1 g. tartaric, 

 0.15 g. malic and 0.26 g. citric acid was 

 used in 65 days by the yeast. Acid de- 

 terminations were made by titration with 

 normal NaOH, using litmus as an indi- 

 cator. 



Added nutrients and variants : 



(a) The author added 9.0 to 10.0% of one 

 of the following organic acids: 

 tartaric malic citric 



(b) Henneberg specified the use of 0.3% 

 KH,P04 and used 0.1% MgS04 in- 

 stead of 0.05%. He added 5.0% glu- 

 cose and used it for the cultivation of 

 lactic acid bacteria. 



Reference: Schukow (1896 p. 607), Henne- 

 berg (1903 p. 7). 



52. Koser's Basal Ammonium Phosphate 



Solution 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. NaCl 5.0 g. 



3. MgS04-7H20 0.2 g. 



