CULTURE MEDIA FOR CULTIVATIOX OF MICRO ORGAXISMS 



19 



4. (NH4)H2P04 1.0 g. 



5. K,HP04 1.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and one of the test 

 materials in 1. Solution is clear at 

 pH = 6.7 to 6.8. 



(2) Add one of the test materials. Add 

 the different organic acids to (1) in 

 such amounts so that when brought to 

 pH reading of 6.8 by addition of 

 NaOH the resulting concentration of 

 the Na salt will be approximately 

 0.2%. 



(3) Tube in 8 to 10 cc. lots. 

 Sterilization: Autoclave at 15 pounds pres- 

 sure for 15 minutes. 



Use: To study the utilization of organic 

 salts and acids by the colon-aerogenes 

 group. Glucose and lactose were added 

 as controls to determine if the solution 

 would support the growth of the organism. 



Added nutrients: The author added ap- 

 proximately 0.2% of the sodium salt of 

 the following acids. In case of citric 

 acid 0.1 to 0.5% was used, 

 glucose 0.2% malic 



lactose 0.2% lactic 



sodmm salt of one of glyceric 



the organic acids citric 



acetic tartaric 



propionic mucic 



n-valeric malonic 



n-butyric oxalic 



iso-valeric benzoic 



n-caproic salicylic 



succinic o-phthalic 



Reference: Koser (1923 p. 497). 



53. Miinter's Basal Ammonium Nitrate 

 Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. MgS04 0.5 g. 



3. NaCl 0.5 g. 



4. CaCl. 0.2 g. 



5. K2HPO4 1.0 g. 



6. NH4NO3 



7. CaCOa 

 Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Neutralize by the addition of CaCOs. 



(3) To each 100.0 cc. of (2) add 0.025 g. 

 of nitrogen in the form of XH4NO3. 



(4) Add 1.0 g. of one of the test materials 

 to (3). 



Sterilization: Not specified. 

 Uses: To study the availability of various 

 carbon sources for Actinomyces odorifer, 

 Act. chromogenes, Act. albus and others. 

 Glycerin laevulose, dextrose, galactose, 

 mannite and starch were good carbon 

 sources for all the actinomycetes studied. 

 The remaining carbohydrates serve as 

 suitable carbon sources for some of the 

 actinomyces but not all. Oxalic acid, 

 tartaric acid, and hippuric acid are not 

 suitable carbon sources for the actinomy- 

 cetes studied. The remaining acids are 

 generally fair carbon sources for most of 

 the actinomycetes studied. 

 Added nutrients: The author used the 

 following carbon sources: 



sucrose glucose 



mannitol arabinose 



galactose laevulose 



lactose glycerol 



inulin starch 



Variants: The author omitted the CaCU 

 and CaCOs and used one of the following 

 carbon sources. The acid was neutral- 

 ized and made slightly alkaline by the 

 addition of Na.COs or CaCOs- 0.025 g. 

 nitrogen in the form of NH4NO3 and 0.25 

 g. of the test material was added to each 

 50.0 cc. of medium. 



oxalic acid zitric acid 



acetic acid hippuric acid 



succinic acid uric acid 



malic acid humus acid 



tartaric acid aspartic acid 



Reference: Miinter (1913 pp. 368, 371). 



54. von Bronsart's Basal Ammonium Nitrate 

 Solution 



Constituents : 



1. Water 1000.00 cc. 



2. iMgS04 (0.25%) 2.5 g. 



3. KH2PO4 (0.25%) 2.5 g. 



4. NH4NO3 (1.0%) 10.0 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Add one of the test materials to (1). 

 Sterilization: Not given. 



Use: To study utilization of various car- 

 bon sources by Xylaria, Xylaria arhua- 

 cula, Xylaria polymorpha, Xylaria hy- 

 poxylon. Growth best using starch as a 

 carbon source with Xylaria arbuscula, 

 and levulose produces best growth with 



