20 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Xylaria polytnorpha, Xylaria hypoxylon 

 growth very poor in any of the solutions. 

 Added nutrients : The author used the fol- 

 lowing carbon sources: 



Starch levulose 



inulin glucose 



dextrin mannitol 



Reference: von Bronsart (1919 p. 57). 



55. Adolf Mayers' Basal Ammonium Nitrate 



Solution (Smith) 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. MgS04 10.0 g. 



3. NH4NO3 15.0 g. 



4. Ca3(P04)2 0.1 g. 



5. Potassium phosphate 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and one of the added 

 nutrients in 1. 



Sterilization: Not specified. 



Use: Study of phosphorescent and acid 

 forming bacteria. 



Variants: Add 3.0% NaCl if luminous bac- 

 teria are to be cultivated, and an excess 

 of pure CaCOs if acid forming bacteria 

 are to be grown. 



Added nutrients : Smith added any desired 

 carbohydrate, alcohol, etc. 



Reference: Smith (1905 p. 197). 



56. Prazmowski's Basal Ammonium Car- 



bonate Solution (Smith) 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. K2HPO4 5.0 g 



3. MgS04 5.0 g. 



4. (NH4)2C03 5.0 g. 



5. CaCl, 0.5 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and one of the added 

 nutrients in 1. 

 Sterilization: Not specified. 

 Use: Study of phosphorescent and acid 



forming bacteria. 

 Added nutrients : Smith added any desired 



carbohydrate, alcohol, etc. 

 Reference: Smith (1905 p. 197). 



SUBGROUP I-B. SECTION 4 



Inorganic liquid media of known composi- 

 tion; nitrogen supplied as ammonium salts; 

 complete nutrient solutions primarily for 

 study of organisms oxidizing ammonia to 

 nitrites. 



Ai.* Ammonia present as ammonium chlo- 

 ride. 

 Hewlett's Ammonium Chloride Solution 



(Johnson) 57 



Beijerinck and Minkman's Ammonium 



Chloride Solution 58 



Aj. Ammonia present as ammonium car- 

 bonate. 

 Prazmowski's Ammonium Carbonate 



Solution. (Tanner) 59 



A3. Ammonia present as ammonium sul- 

 phate. 

 Bi. Without additional salts. 

 Wimmer's Ammonium Sulphate Solu- 

 tion 60 



B2. With other salts. 

 Ci. Magnesium carbonate added. 

 Di. Phosphates not added. 

 Winogradsky's Ammonium Sulphate 



Solution (Harvey) 61 



D2. Phosphates added. 

 El. All additional salts of mono valent 

 cations. 

 Buhlert and Fickenday's Ammonium 



Sulphate Solution 62 



E,i. Additional salts of mono and di valent 



cations. 

 Fi. Calcium salts present. 

 Winogradsky's Ammonium Sulphate 



Solution 63 



Giltner's Modified Winogradsky's Am- 

 monium Sulphate Solution 64 



F2. Calcium salts not present. 

 Winogradsky and Omeliansky's Am- 

 monium Sulphate Solution 65 



E3. Containing Additional salts of tri- 

 valent cations. 

 Gibbs' Ammonium Sulphate Solution. 



(modified by Gowda) 68 



C2. Magnesium carbonate not added. 

 Di. Calcium carbonate, chalk or lime 



added. 

 El. Magnesium sulphate added. 



Omeliansky's Ammonium Sulphate 



Solution. (Harvey) 67 



E2. Magnesium sulphate not added. 

 Christensen's Ammonium Sulphate 



Solution 68 



D2. Calcium carbonate chalk or lime not 

 added. 



Gage's Ammonium Sulphate Solu- 

 tion 69 



See A2 A3 and A4. 



