CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



21 



Omeliansky's Ammonium Sulphate 



Solution. (Lohnis-Arnd) 70 



Lolinis' Ammonium Sulphate Solu- 

 tion 71 



A4. Ammonia present as a salt of phos- 

 phoric acid. 



Stutzer's Ammonium Magnesium 

 Phosphate Solution 72 



57. Hewlett's (Johnson) Ammonium Chlo- 



ride Solution. 



Constituents : 



1. Distilled water 1000.0 cc. 



2. NH4CI 0.5 g. 



3. Potassium phosphate 0.1 g. 



4. MgS04 0.02 g. 



5. CaCl. 0.01 g. 



6. CaCOs 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add 6 and distribute into flasks. 

 Sterilization: Not specified. 



Use: For the study of nitrite formation. 

 Reference: Johnson (1912 p. 219). 



58. Beijerinck and Minkman Ammonium 



Chloride Solution 



Constituents: 



1. Water 100.0 cc. 



2. K2HPO4 0.02 g. 



3. NH4CI 0.02 g. 



4. NaHCOs 0.1 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Distribute into Sohngen's culture 

 apparatus. 



Sterilization: Not specified. 



Use : Inoculate with soil for study of forma- 

 tion of nitrites. Cultivation of Bacillus 

 sausseri. 



Reference: Beijerinck and Minkman 

 (1910 p. 60). 



59. Prazmowski's Ammonium Carbonate 



Solution (Tanner) 



Constituents : 



1. Distilled water 1000.0 cc. 



2. K2HPO4 5.0 g. 



3. MgS04-7H20 5.0 g. 



4. (NH4)2C03 5.0 g. 



5. CaCla 0.5 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization : Not specified. 

 Reference: Tanner (1919 p. 63). 



60. Wimmer's Ammonium Sulphate 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. (NH4)2S04 2.0 or 4.0 g. 



Preparation: (1) Dissolve 2 in 1. 

 Sterilization: Not specified. 



Use: For study of nitrification (production 



of nitrites) by soil bacteria. 

 Reference: Wimmer (1904 p. 140). 



61. Winogradsky Ammonium Sulphate 

 Solution (Harvey) 



Constituents : 



1. Distilled water 1000.0 cc. 



2. (NH4)2S04 1.0 g. 



3. K2SO4 1.0 g. 



4. MgS04 7.5 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization : Not specified. 



Use: Study of nitrifying organisms. 

 Reference: Harvey (1921 p. 106). 



62. Buhlert and Fickendey Ammonium 

 Sulphate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. (NH4)2S04 4.0 g. 



3. Potassium phosphate 2.0 g. 



4. Basic magnesium carbonate 

 (4MgC03-Mg(OH)2-5H20).. 40.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 



Sterilization: Not specified. 



Use: Study of nitrifying bacteria of the 



soil. 

 Variants : 



(a) Fremlin's (described by Gage) solu- 

 tion is simifar; the potassium phos- 

 phate is specified as KH2PO4, and the 

 proportions as follows: 



1. Water (nitrite free) 1100.0 g. 



2. KH2PO4 1.0 g. 



3. (NH4)2S04 1.0 g. 



4. MgC03 1.0 g. 



2 and 3 dissolved in 1000.0 cc. water 

 and 4 in 100.0 cc. water. These solu- 

 tions are sterilized separately and 

 mixed aseptically. 



(b) Percival distributed the solution in 

 25.0 cc. lots, and added 20.0 cc. of a 

 mixture of equal parts soil and sterile 

 tat) water to each lot. 



