22 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(c) Abbott gave the following solution: 



1. Water 1000.0 cc. 



2. (NH4)2S04 1.0 g. 



3. Potassium phosphate... 1.0 g. 



4. MgCOs (Basic) 



(1) Dissolve 2 and 3 in 1. 



(2) Flask in 100.0 cc. quantities. 



(3) Add 0.5 to 1.0 g. of basic MgCOs sus- 

 pended in a little sterile water to 

 each flask. 



References: Buhlert and Fickendey (1906 

 p. 404), Barthel (1910 p. 112), Percival 

 (1920 p. 208), Gage (1910 p. 14), Abbott 

 (1921 p. 603). 



63. Winogradsky's Ammonium Sulphate 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. Potassium phos- 

 phate 1 g. 



3. MgS04 0.5 g. 



4. CaCla trace 



5. MgCOs amount not specified 



6. (NH4)2S04 2.0 to 2.5 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Distribute in flasks. 



(3) After sterilization add MgCOs 

 (amount not specified) and from 2 to 



2.5 parts per thousand of (NH4)2S04. 

 Sterilization: Method not specified. 

 Use: To show nitrification by organisms 



found in the soil. Inoculate with soil. 

 Variants : 



(a) Burri and Stutzer 



1. Water 1000.0 cc. 



2. NaCi 0.5 g. 



3. KH2PO4 ' 1.0 g. 



4. MgS04 0.5 g. 



5. CaCU trace 



6. (NH4)2S04 2.0 g. 



7. MgCOs 



(b) Heinemann 



1. Water 1000.0 cc. 



2. Potassium phosphate 1.0 g. 



3. MgS04 0.5 g. 



4. CaCla 0.01 g. 



5. NaCl 2.0 g. 



6. MgCOs 



7. (NH4)2S04 2.0 g. 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Distribute in 20.0 cc. lots. 



(3) Add several tenths grams of MgCOj 

 to each lot. 



(4) Sterilize intermittently. 



(5) Add 1.0 or 2.0 cc. of a sterile 2.0% 

 (NH4)2S04 solution to each lot under 

 aseptic conditions. 



(c) Harvey modified Burri and Stutzer's 



solution in that he used 1.0 g. 



K2HPO4 instead of KH2PO4 and used 



2.0 g. NaCl instead of 0.5 g. 



References: Winogradsky (1891 p. 577), 



Burri and Stutzer (1896 p. 106), Harvey 



(1921-22 p. 105) Heinemann (1905 p. 129). 



64. Giltner's Modified Winogradsky's 

 Ammonium Sulphate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. (NH4)2S04 0.4 g. 



3. MgS04 0.05 g. 



4. K2HPO4 0.1 g. 



5. NaoCOs 0.6 g. 



6. CaClo trace 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: Study of nitrate formation. 

 Reference: Giltner (1921 p. 369). 



65. Winogradsky and Omeliansky's 



Ammonium Sulphate Solution 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. (NH4)2S04 2.0 g. 



3. Potassium phosphate 1-0 g. 



4. MgS04 0.5 g. 



5. NaCl 2.0 g. 



6. FeS04 0.4 g. 



7. MgCOs in excess 



Preparation : 



(1) Dissolve 3, 4, 5 and 6 in 1. 



(2) Distribute in 50.0 cc. lots. 



(3) Add MgCOs in excess to each flask. 



(4) After sterilization add 1.0 cc. of 

 a sterile 1.0% solution of pure 

 (NH4)2S04 under aseptic conditions. 



Sterilization : Method not specified. 



Use : To study nitrification by soil bacteria. 



Variants: The appended table gives com- 

 binations used by various authors. The 

 preparation of these variants is practi- 

 cally the same as for the original. Omeli- 

 ansky found that repeated inoculation 

 from this medium into the same medium 

 usually yielded a pure culture of nitrify- 



