CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



23 



ing organisms when initially inoculated 

 with soil. Giltner and Ldhnis used 

 Harvey's solution (1.0 g. (NH4)2S04) and 

 suggested the use of either CaCOa or 

 MgCOs. He reported that both nitrite 

 and nitrate bacteria are stimulated if 

 CaCOs is used instead of MgCOs. Tan- 

 ner used KH2PO4 in Harvey's (a) solution 

 instead of K2HPO4. 



(3) Distribute (1) in 5.0 cc. lots. 



(4) Add 0.5 cc. of (2) to each 5.0 cc. lot 

 of (3). 



Sterilization: Not specified. 



Use: To isolate nitrite formers from the 

 soil and to study nitrification. Tromms- 

 darf's reagents were used to test for the 

 presence of nitrites. 



Reference: Gowda (1924 p. 252). 



INVESTIGATOR 



Distilled water 



(NH4)2S04 



NaCl 



Potassium phosphate 



MgS04 



FeS04 



MgCO, 



K2HPO4 



KH2PO4 



KCl 



References : Winogradsky and Omeliansky 

 (1899 p. 432), Omeliansky (1899 p. 539), 

 Smith (1905 p. 199), BouUanger and 

 Massol (1903 p. 493), Wimmer (1904 p. 

 139), Lohnis (1913 p. 97), Percival (1920 

 p. 144), Harvey (1921-22 p. 105), Giltner 

 (1921 p. 375), Tanner (1919 p. 67), Cun- 

 ningham (1924 p. 150). 



66. Gibbs' Ammonium Sulphate Solution 

 (modified by Gowda) 



Constituents : 



1. Conductivity water 1100.0 cc. 



2. (NH4).2S04 l.Og. 



3. K2HPO4 l.Og. 



4. NaCl 2.0 g. 



5. MgS04 0.5 g. 



6. Fe2(S04)3 trace 



7. MgCO, 5.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1000.0 cc. 

 conductivity water. 



(2) Dissolve 7 in 100.0 cc. of conductivity 

 water. 



67. 



Omeliansky Ammonium Sulphate Solu- 

 tion (Harvey) 



Constituents: 



1. Water 



2. K.2HPO4 



3. MgS04 



4. (NH4)2S04 



5. NaCl 



6. Precipitated chalk 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add 6 to (1). 



(3) Distribute in flasks. 

 Sterilization: Not specified. 

 Use: Study of nitrifying bacteria. 

 Variants: Niklewski used the following 



solution to study nitrification by bacteria 

 from stable manure. It was prepared 

 the same as the above solution. 



1. Water 1000.0 cc. 



2. (NH4)2S04 (0.05%) 0.5 g. 



3. K2HPO4 (0.02%..) 0.2 g. 



4. MgS04 (0.01%) 0.1 g. 



