CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



25 



1. Water 1000.0 cc. 



2. K2HPO4 10 g. 



3. NaCl 0.25 g. 



4. FeS04 0.025 g. 



5. Ammonium magnesium 

 phosphate (drj-) 20.0 g. 



6. MgCOs 20.0 g. 



The medium was prepared by mixing 5 



and 6 in a mortar and adding a teaspoon- 

 f ul or more of the mixture to flat bottomed 

 flasks. Then add 50.0 to 100.0 cc. of a 

 solution of 2, 3 and 4 in 1 to each flask. 

 Sterilize the flasks. Method not given. 

 Reference: Stutzer (1901 p. 173). 



SUBGROUP I-B. SECTION 5 



Inorganic liquid media of known com- 

 position, nitrogen supplied as ammonium 

 salts, complete nutrient solutions, but not 

 used primarily for organisms oxidizing 

 ammonia to nitrites. 



A few media have been described contain- 

 ing ammonium salts but no organic carbon 

 for the cultivation of microorganisms which 

 do not secure their growth energy by oxida- 

 tion of ammonia. These media are of two 

 general classes, those used with sulphur or 

 its compounds for the cultivation of the 

 bacteria oxidizing sulphur, and those for 

 other types of organisms, including the 

 algae. The various media may be differen- 

 tiated as follows: 



Ai. Primarily for organisms oxidizing sul- 

 phur or its compounds. 

 Waksman's Ammonia and Sulphur 



Solution no. 2 73 



Waksman's Ammonia and Sulphur 



Solution 74 



Waksman's Sulphide Solution 75 



Beijerinck's Thiosulphate Solution.... 76 

 A2. Not primarily for organisms oxidizing 



sulphur. 

 Bi. Ammonia supplied as ammonium 

 chloride. 



Kaserer's Hydrogen Ammonium Chlo- 

 ride Solution 77 



Beijerinck and van Deiden's Ammo- 

 nium Chloride Solution 78 



B2. Ammonia supplied as ammonium 

 nitrate. 

 Richter's Ammonium Nitrate Solu- 

 tion 79 



B3. Ammonia supplied as ammonium sul- 

 phate. 



Lieske's Ammonium Sulphate Solu- 

 tion 80 



Gottheil's Ammonium Sulphate Solu- 

 tion. (No. XI) 8. 



Gosling's Ammonium Sulphate Solu- 

 tion 82 



Beijerinck and van Deiden's Ammo- 

 nium Sulphate Solution 83 



B4. Ammonia supplied as a salt of phos- 

 phoric acid. 



Beijerinck and van Deiden's Manga- 

 nese Ammonium Phosphate Solu- 

 tion 84 



Beijerinck and van Deiden's Ammo- 

 nium Phosphate Solution 85 



73. Waksman's Ammonia and Sulphur 

 Solution No. 2 



Constituents : 



1. Distilled water 1000.0 cc, 



2. (NH4)2S04 0.2 g. 



3. iMgS04-7H20 0.5 g. 



4. KH2PO4 1.0 g. 



5. Ca3(P04)2 (re-precipitated) 2.5 g. 



6. Sulfur 10.0 g. 



7. H3PO4 

 Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Weigh sulfur and Ca3(P04)2 sepa- 

 rately into the individual flasks into 

 which the medium is distributed 

 (100.0 cc. portions are usuall}' placed 

 in 250 cc. flasks). 



(3) Adjust to pH = 3.0 by the addition of 

 M/1 H3PO4. 



Sterilization : Sterilize in flowing steam for 



30 minutes on 3 successive days. 

 Use: Isolation and cultivation of Thio- 

 bacillus thiooxidans. 

 Reference : Waksman (1922 p. 606). 



74. Waksman's Ammonia and Sulphur 

 Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. (NH4)2S04 0.2 g. 



3. MgS04-7H20 0.5 g. 



4. KH2PO4 3.0 g. 



5. CaCla 0.25 g. 



6. Sulphur (powdered) 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Weigh out the sulphur separately in 

 the individual flasks into which the 



