CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



27 



(4) Prepare hydrogen gas from c.p. 

 H2SO4 and zinc. 



(5) Pass hydrogen and carbonic acid into 

 the flask. 



Sterilization: Not specified. 



Use: To show oxidation by soil forms, 

 Bacillus oligocarbophilus and Bacillus 

 methylicus . After 5 days the amount of 

 hydrogen was less in the flask inoculated 

 with soil than in the control flask. 



Variants : Lantzsch using the same solution 

 found that Actinomyces (Bacillus oligo- 

 carbophilus oxidized hydrogen in a non- 

 sterilized medium, but failed in a steril- 

 ized medium. 



Reference: Kascrer (1906 p. 686), Lantzsch 

 (1922 p. 316). 



78. Beijerinck and van Delden's Ammonium 



Chloride Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. NH4CI 0.1 to 1.0 g. 



3. Potassium phosphate 0.2 g. 



4. Beijerinck and van Delden's basic salt 

 solution (see p. 12) 10 drops. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add 1.0 drop of Beijerinck and van 

 Delden's basic salt solution (see page 

 12) to each 100.0 cc. of (1). 



Sterilization: Not specified. 



Use: Cultivation of Bacillus oligocarbo- 

 philus. 



Reference: Beijerinck and van Delden 

 (1903 p. 36). 



79. Richter's Ammonium Nitrate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. NH4NO3 3.0 g. 



3. MgS04 1.0 g. 



4. KH2PO4, FeS04 and 



ZnS04 1.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 

 Use: Growth oi Aspergillus niger. 

 Reference: Richter (1910 p. 619). 



80. Lieske's Ammonium Sulphate Solution 



Constituents: 



1. Water 



1000.0 cc. 



2. Magnesium bicarbonate 



3. NaHC03 (0.001%) 0.01 g. 



4. (NH4)2S04 (0.001%) 0.01 g. 



5. Potassium phosphate trace 



6. MgS04 

 Preparation : 



(1) Prepare Magnesium bicarbonate by 

 adding pure carbonic acid to a sus- 

 pension of Magnesium carbonate. 



(2) Filter. 



(3) Dilute the filtrate 1 to 10 with water. 



(4) Dissolve 3, 4, 5 and 6 in (3). 

 Sterilization: Not specified. 



Use: Cultivation oi Leptothrix ochracea. 

 Reference: Lieske (1919 p. 422). 



81. Gottheil's Ammonium Sulphate Solution 



(No. XI) 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 1.0 g. 



3. CaCla 0.1 g. 



4. MgS04 0.3 g. 



5. NaCl 0.1 g. 



6. Iron trace 



7. (NH4)2S04 0.25 mg. 



8. NaoCOa 0.5 g. 



Preparation: (1) Dissolve 2, 3, 4, 5, 6, 7 



and 8 in 1. 

 Sterilization: Not specified. 

 Use : Cultivation of organisms found in the 



soil on roots and rhizomes. 

 Reference: Gottheil (1901 p. 432). 



82. Gosling's Ammonium Sulphate Solution 



Constituents: 



1. Distilled water 500.0 cc 



2. NazCOs (dehydrated) 2.3 g 



3. CaCOs 0.25 g 



4. K.,C03 0.08 g 



5. FeS04 0.03 g 



6. (NH4)2S04 0.005 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 

 Sterilization: Not specified. 

 Use: To determine sulphate reduction. 

 Reference: Gosling (1904 p. 391). 



83. Beijerinck and van Delden's Ammonium 



Sulphate Solution 



Constituents: 



1. Distilled water 1000.0 cc. 



2. (NH4)2S04 1.0 g. 



3. Potassium phosphate 0.2 g. 



