28 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



4. Beijerinck and van Del- 

 den's mineral solution (see 

 p. 12). 

 Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add 1.0 drop of 4 to each 100.0 cc. 

 of (1). 



Sterilization: Not specified. 

 Use: Growth of Bacillus oligocarbophilus. 

 Reference: Beijerinck and van Delden 

 (1903 p. 36). 



84. Beijerinck and van Delden's Manganese 



Ammonium Phosphate Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. (NH4)2HP04 0.2 g. 



3. NaaCOa 1.0 g. 



4. Beijerinck and van Del- 

 den's solution (see p. 12). . 20 drops. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add 2.0 drops of 4 to each 100.0 cc. 

 of (1). 



Sterilization: Not specified. 



Use: Bacillus oligocarbophilus grows as a 



snow white scum on the surface of the 



medium. 

 Reference: Beijerinck and van Delden 



(1903 p. 41). 



85. Beijerinck and van Delden's Ammo- 



nium Phosphate Solution 



Constituents : 



1. Distilled water 100.0 cc. 



2. K2HPO4 0.02 g. 



3. (NH4)2HP04 0.02 g. 



4. NazCOa 0.1 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) Reaction to be slightly alkaline. 

 Sterilization: Not specified. 



Use: Bacillus oligocarbophilus grows as a 

 thin white scum on the medium. 



Reference: Beijerinck and van Delden 

 (1903 p. 41). 



SUBGROUP I-B. SECTION 6 



Inorganic liquid media or basal solutions 

 of known composition; nitrogen supplied 

 as nitrites. 



The several liquid media which have 

 been published for the growth of organisms 



oxidizing nitrites may be differentiated as 



follows: 



A I. Employed primarily for organisms 

 oxidizing nitrites to nitrates. 



Bi. Calcium carbonate added. 

 Hewlett's Nitrite Solution (Johnson). 86 

 Cunningham's Nitrite Solution 87 



B2. Calcium carbonate not added. 



Ci. Magnesium carbonate added. 



Omeliansky's Nitrite Solution 88 



C2. Magnesium carbonate not added. 



Di. Magnesium sulphate added. 



Winogradsky and Omeliansky's So- 

 dium Nitrite Solution 89 



Winogradsky 's Basal Sodium Nitrite 

 Solution 90 



D2. Magnesium sulphate not added. 

 Gartner's Sodium Nitrite Solution ... 91 

 Wimmer's Sodium Nitrite Solution.. . 92 

 Bijerinck and van Delden's Basal 

 Nitrite Solution 93 



A2. Not employed primarily for organisms 

 oxidizing nitrites to nitrates. 

 Gottheil's Nitrite Solution No. VIII . 94 

 Bijerinck and van Delden's Potassium 

 Nitrite Solution A 95 



86. Hewlett's Nitrite Solution (Johnson) 



Constituents : 



1. Distilled water 1000.0 cc. 



2. KNO2 0.3 g. 



3. Potassium phosphate 0.1 g. 



4. MgS04 0.05 g. 



5. CaCOs 5.0 g. 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use: Study of nitrate production. Bacil- 

 lus megatherium produced no nitrates 

 in this solution. 



Reference: Johnson (1912 p. 219). 



87. Cunningham's Nitrite Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. NaNOo 1.0 g. 



3. K2HPO4 0.5 g. 



4. MgS04 0.3 g. 



5. NaCl 0.5 g. 



6. Na.COa 0.2 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Distribute in 10.0 cc. quantities in 

 50.0 cc. Erlenmeyer flasks. 



