30 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



(3) Adjustment of reaction not specified. 



(4) Flask in 50.0 cc. lots. 

 Sterilization: Sterilize in the autoclave. 

 Use: To study nitrification. 



Added nutrients and variants : The author 

 added glucose, urea, asparagin or peptone 

 to the solution to determine the efTect of 

 organic materials on nitrification. Dis- 

 tribute in 50.0 cc. lots and add: 



(a) 0.2, 0.4, 0.8, 1.0, 1.2, 1.6, 2.0, 2.4 or 

 4.0 g. glucose. 



(b) 0.05, 0.1, 0.2, 0.4, 0.8, 1.2 or 1.6 g. 

 peptone. 



(c) 0.1, 0.2, 0.4 or 0.8 g. urea. 



(d) 0.1, 0.2 or 0.4 g. asparagin. 



(e) 5.0 cc. of bouillon. 



The presence of organic materials gen- 

 erally tends to slow up the oxidation. 



3. K2CO3 5.0 g. 



4. NaNOs 2.0 g. 



Preparation : 



(1) Dissolve 2, 3, and 4 in 1. 



(2) Distribute in 30 cc. lots in flasks. 

 Sterilization: Not specified. 



Use: To study the oxidation of nitrite by 

 fungus. Nitrite was found to be still 

 present after four weeks. 



Reference: Gartner (1898 p. 4). 



92. Wimmer's Sodium Nitrite Solution 



Constituents: 



1. Distilled water.. 1000.0 cc. 



2. Natrium nitro- 

 sum puriss 



(NaNOo) 1.0, 2.0 or 50.0 g. 



Preparation: (1) Dissolve 2 in 1. 



Distilled water 



NaN02 



Potassium phosphate 



MgS04 



NaCl 



NaoC03 



K2HPO4 



K2CO3 



KCl 



WINOGRADSKY 

 AND 



OMELIANSKY 



1000.0 cc. 

 1.0 g. 



0.5 g. 

 0.3 g. 

 0.5 g. 

 1.0 g. 



1000.0 cc. 

 2.0 g. 



0.3 g. 

 0.5 g. 



1.0 g. 

 0.5 g. 



1000.0 cc. 



25.0 cc. 2.0% 



solution 



1.0 g. 



0.5 g. 



l.Og. 



0.1 



1000.0 cc. 

 l.Og. 



0.3 g. 

 0.5 g. 

 0.3 g. 

 l.Og. 



Peptone in small amounts tends to 

 hasten oxidation. Same is true of urea. 

 Asparagin and glucose even in small 

 amounts slow up the oxidation. 

 Variants: The modifications shown in the 

 table have been described. Their prepa- 

 ration is like the original solution. 

 Stutzer and Hartleb bubbled air con- 

 taining CO2 thru their solution. Carbo- 

 nates or CO 2 were essential for nitrate 

 production. 

 References: Winogradsky (1896 p. 423), 

 Stutzer and Hartleb (1897 p. 244), Wino- 

 gradsky and Omeliansky (1899 p. 378), 

 Stutzer (1901 p. 169), Lohnis (1913 p. 97) 

 Giltner (1921 p. 375). 



91. Gartner's Sodium Nitrite Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Potassium phosphate 0.5 g. 



Sterilization: Not specified. 



Use : To study nitrification by soil bacteria. 



Raference: Wimmer (1904 p. 140). 



93. Beijerinck and van Delden's Basal 

 Nitrite Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KNO2 0.5 g. 



3. K0HPO4 0.5 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add one of the added nutrients to (1). 

 Sterilization: Not specified. 



Use: Tostudy denitrification by 5. subtilis, 

 B. mesenlericus and Azo. chrooincoccum. 

 B. subtilis gave no ammonia test. B. 

 mesenlericus gave ammonia using man- 

 nitol as a carbon source. Chroococcum 

 gave ammonia using either mannitol or 

 malate as a carbon source. 



