CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



31 



Added nutrients: The authors used either 

 20.0 g. of mannitol or 20.0 g. calcium 

 malate as a carbon source. 



Reference: Beijerinck and van Delden 

 (1902 p. 41). 



94. Gottheil's Nitrite Solution No. VIII 



Constituents. 



1. Distilled water 1000.0 cc. 



2. Potassium phosphate 1-0 g. 



3. CaClo 0.1 g. 



4. MgSOi 0.3 g. 



5. NaCl 0.1 g. 



6. Iron trace 



7. KNO2 0.05 g. 



8. Soda 0.05 g. 



Preparation : Dissolve 2, 3, 4, 5, 6, 7 and 8 



in 1. 

 Sterilization: Not specified. 

 Use: Cultivation of bacteria found in soil 



on roots and rhizomes. 

 Reference: Gottheil (1901 p. 432). 



95. Beijerinck and van Delden's Potassium 

 Nitrite Solution A 



Constituents: 



1. Water (tap) 100.0 cc. 



2. KCl 0.01 g. 



3. K2HPO4 0.02 g. 



4. Kx\02 0.01 g. 



Preparation : 



(1) Dissolve 2, 3 and 4 in 1. 



(2) The reaction is slightly alkaline. 

 Sterilization: Not given. 



Use: Bacillus oligocarbophilus grows in a 

 dry thin snow white scum on the surface 

 of the medium. 



Reference: Beijerinck and van Delden 

 (1903 p. 41). 



SUBGROUP I-B. SECTION 7 



Inorganic basal solutions of known com- 

 positions; nitrogen supplied as nitrates; 

 incomplete solutions requiring the addition 

 of other nutrients. 

 A I. Saltsof monovalent cations only added. 



Beijerinck's Basal Nitrate Solution. 96 



Stoklasa and Vitek's Basal Nitrate 



Solution 97 



A2. Salts of mono and divalent cations 



added. 



Czapek's Basal Nitrate Solution 

 (Waksman) 98 



Giltay-Aberson's Basal Nitrate Solu- 

 tion (Stoklasa) 99 



Henneberg's Basal Nitrate Solution. 100 

 A3. Salts of mono, di and tri valent cations 



added. 

 Bi. Calcium salts added. 



Stoklasa and Vitek's Basic Nitrate 

 Salt Solution 101 



Harvey's Basal Nitrate Solution 102 



Bo. Calcium salts not added. 



Beijerinck and van Delden's Nitrate 

 Solutions, B, E, F, H, N 103 



Kita's Basal Nitrate Solution 104 



Stoklasa's Basal Nitrate Solution . . . 105 



Lantzsch's Basal Nitrate Solution. . . 106 



96. Beijerinck's Basal Nitrate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. KNO3 1.0 g. 



3. K2HPO4 0.2 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Add one of the materials listed under 

 added nutrients. 



Sterilization: Not specified. 



Use: To study denitrification by Azoto- 



bacter chroococcum. 

 Added nutrients and variants : 



(a) The author added 20.0 to 100.0 g. 

 mannitol. 



(b) Beijerinck and van Delden used 0.2 g. 

 KNO3 in the basic solution without 

 additional nutrients for the cultiva- 

 tion of Bacillus oligocarbophilus . 



(c) Beijerinck and van Delden used 0.2 g . 

 KNO3 and 0.2 g. Na2HP04 in the 

 basic solution and added 0.2 g. KCl for 

 the cultivation of Bacillus oligocarbo- 

 philus. 



(d) Beijerinck and van Delden used 0.1 g. 

 KNO3 and 0.1 g. K2HPO4 in the basic 

 solution and added 0.1 g. Na2C08 for 

 the cultivation of Bacillus oligo- 

 carbophilus. 



(e) Beijerinck and Minkman used 1.0% 

 KNO3 and 0.05% K.2HPO4 in the 

 basic solution and added 2.0% man- 

 nitol, glycerol, sodium acetate or 

 sodium propionate to study deni- 

 trification. 



(f ) Beijerinck and van Delden used 0.5 g. 

 KNO3 and 0.5 g. K2HPO4 in the basic 

 solution and added 2.0 g. of calcium 



