32 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



malate or mannitol to study deni- 



trification. 

 References: Beijerinck (1901 p. 575), Beij- 

 erinck and van Delden (1902 p. 41), 

 (1903 p. 41), Beijerinck and Minkman 

 (1910 pp. 35, 36). 



97. Stoklasa and Vitek's Basal Nitrate 



Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. NaNOs 8.5 g. 



Preparation : 



(1) Dissolve 2 in 1. 



(2) Add a suitable carbon source. 

 Sterilization: Not specified. 



Use: To study nitrate reduction by Bad. 



Hartlebi and other bacteria. 

 Added nutrients : The author suggested the 



addition of the following carbon sources: 



xylose 15.0 g. 



glucose 18.0 g. 



sucrose 34.2 g. 



Reference: Stoklasa and Vitek (1905 p. 



205). 



98. Czapek's Basal Nitrate Solution 

 (Waksman) 

 Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. MgS04 0.5 g. 



4. KCl 0.5 g. 



5. FeS04 0.01 g. 



6. NaNOa 2.0 g. 



Preparation : 



(1) Prepare a solution of 1, 2, 3, 4, 5 and 6 

 using chemically pure materials. 



(2) Add 20.0 g. of one of the test ma- 

 terials. 



Sterilization: Autoclave at 15 pounds pres- 

 sure for 15 minutes. 



Use: To study the metabolism of actino- 

 mycetes. The author found that the 

 Actinomycetes utilized the materials in 

 order listed. Starch, glucose, lactose, 

 maltose, glycerin, sucrose, cellulose and 

 then organic acids. 



Added nutrients: The author used 20.0 g. 

 of one of the following carbon sources: 

 arabinose starch 



glycerol sodium salts of : 



lactose acetic acid 



sucrose malic acid 



maltose sodium salts of: 



mannitol tartaric acid 



inulin oxalic acid 



glucose lactic acid 



cellulose in form of Whitman filter paper 

 1.0 cc. of 0.5% cellulose suspension. 

 Reference: Waksman (1919 p. 317). 



99. Giltay-Aberson's Basal Nitrate Solution 



(Stoklasa) 

 Constituents: 



1. Water 1000.0 cc. 



2. NaNOs 2.0 g. 



3. MgS04 2.0 g. 



4. K2HPO4 2.0 g. 



5. NaCl 0.2 g. 



6. Iron chloride less than 0.2 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Adjust to slightly alkaline reaction 

 by adding Na2C03. 



(3) Add one of the combinations given 

 in added nutrients. 



Sterilization: Not specified. 



Use: To study denitrification and influence 



of carbohydrates on denitrification 



by soil forms. 

 Added nutrients: The author added one of 



the following combinations: 



(a) Citric acid 5.0 g. and glucose 2.0 g. 



(b) Glucose 2.0 g. 



(c) Glucose 2.0 g. with stable manure. 



(d) Glucose 2.0 g. and citric acid 5.0 g. 

 with stable manure. 



(e) Glucose 2.0 g. with Chilisaltpeter. 



(f) Glucose 2.0 g. and citric acid 5.0 g. 

 with Chilisaltpeter. 



(g) Chilisaltpeter, peat, or soil rich in 

 humus. 



Reference: Stoklasa (1907 p. 28). 



100. Henneberg's Basal Nitrate Solution 



Constituents: 



1. Water 1000.0 cc. 



2. MgS04 1.0 g. or 2.0 g. 



3. KNO3 2.0 g. or 3.0 g. 



Preparation: 



(1) Dissolve 2 and 3 in 1. 



(2) Add one of the test materials to (1). 

 Sterilization: Not specified. 



Use: To determine the fermentation of 

 acetic acid bacteria, B. oxydans, B. 

 acetosum, B. aceti, B. Kutzingianum, B. 

 Pasteurianum, B. acetigenum. B. oxy- 



