CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



33 



dans ferments arabinose, levulose, dex- 

 trose, galactose, maltose, dextrin, ethyl 

 and propyl alcohol, erythritol. B. aceto- 

 sum ferments arabinose, dextrose, galac- 

 tose, ethyl and propyl alcohol. B. aceti, 

 B. Kiitzingianum, B. Pasteurianum and 

 B. acetigenum ferment dextrose and 

 ethyl and methyl alcohol. 

 Added nutrients: The author added the 

 following carbon sources: 

 arabinose inulin 



levulose methyl alcohol 



glucose ethyl alcohol 



galactose propyl alcohol 



sarobose amyl alcohol 



sucrose glycerol 



maltose erythritol 



lactose mannitol 



dextrin dulcitol 



starch melampyrit 



glycogen quercitol 



Reference: Henneberg (1898 p. 19). 



101. Stoklasa and Vitek's Basal Nitrate 

 Salt Solution 



Constituents: 



1. Water 1000.0 cc 



2. NaNOs 2.0 g. 



3. K2HPO4 1.25 g. 



4. K2SO4 0.2 g. 



5. CaCl2 0.05 g. 



6. MgCU 0.05 g. 



7. NaiCOs 0.1 g. 



8. FeP04 0.05 g. 



Preparation : 



(1) Prepare solutions as indicated in the 

 table by dissolving the salts in water. 



(2) Add 2.0 or 2.5 g. of test material. 

 Sterilization: Not specified. 



Use: To study reduction of nitrates by 



denitrifying organisms. 

 Added nutrients: The author added a 



variety of carbohydrates (2.0 o;- 2.5 g.) 



or 2.0 g. of organic acids. Neutralize 



the acids with NaoCOs. 



butj-ric acid glucose 



succinic acid levulose 



lactic acid galactose 



citric acid arabinose 



malic acid xjdose 



tartaric acid saccharose 



asparagin lactose 



Variants: The authors substituted 0.25 g. 



Na2HP04 for 1.25 g. K2HPO4. 



Reference: Stoklasa and Vitek (1905 p. 

 104) (1901 p. 262). 



102. Harvey's Basal Nitrate Solution 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. NaNOa 1.0 g. 



3. NaCl 0.02 g. 



4. MgS04 0.02 g. 



5. K2HPO4 0.5 g. 



6. FeCla trace 



Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) Add one of the added nutrients. 

 Sterilization: Not specified. 



Use: Study of nitrate reduction. 



Added nutrients: The author added 10.0 g. 



glucose or 10.0 g. glycerol. 

 Variants : The author used the following 



solution: 



1. Distilled water 3000.0 cc. 



2. KNO3 7.0 g. 



3. K0HPO4 1.5 g. 



4. MgS04 1.5 g. 



5. NaCl 1.5 g. 



6. CaS04 5.0 g. 



7. FeCl 3 solution few drops 



Reference: Harvey (1921-22 p. 104). 



103. Beijerinck and van Delden's Nitrate 

 Solution B, E, F, H, N 



Constituents : 

 Solution B: 



1. Distilled water 1000.0 cc. 



2. KCl 0.2 g. 



3. KNO., 1.0 g. 



4. K2HPO4 0.2 g. 



Solution E: 



1. Distilled water 1000.0 cc, 



2. KCl 0.1 g. 



3. KNO3 1.0 g. 



4. K2HPO4 0.2 g. 



Solution F: 



1. Distilled water 1000.0 cc. 



2. KNO3 0.1 g. 



3. Na2HP04 0.2 g. 



Solution H: 



1. Distilled water 1000.0 cc. 



2. KCl 0.2 g. 



3. KNO3 0.2 g. 



4. Na2HP04 0.2 g. 



Solution N: 



1. Distilled water 1000.0 cc. 



2. KNO3 0.1 to 1.0 g. 



3. K2HPO4 0.1 g. 



