34 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



Preparation : 



(1) Dissolve the salts in water as indi- 

 cated in the table. 



(2) Add one drop of Beijerinck and van 

 Delden's mineral solution (see Med. 

 25) to each 100.0 cc. of (1). 



Sterilization: Not specified. 



Use: Bacillus oligocarbophilus grows as a 



dry thin snow white scum on the surface 



of the medium. 

 Reference: Beijerinck and van Delden 



(1903 p. 41). 



lOi. Kita's Basal Nitrate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. MgS04 0.25 g. 



3. KH2PO4 5.0 g. 



4. FeCls solution few drops 



5. KNO3 50 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add 5.0% of any carbohydrate. 



(3) Adjustment of reaction not specified. 

 Sterilization: Not specified. 



Use: Cultivation of molds, Aspergillus 

 albus, Aspergillus Okazaki, Aspergillus 

 candidus, Aspergillus tamarii, Pseudo- 

 rhizopus, Aspergillus glaucus. 



Added nutrients: The author did not 

 specify the carbohydrates used. 



Reference: Kita (1913 p. 434). 



105. Stoklasa's Basal Nitrate Solution 



Constituents : 



1. Distilled water 1000.0 cc. 



2. K2SO4 10 g. 



3. MgCl2 0.5 g. 



4. Iron sulphate 0.1 g. 



5. NaNOa 



6. Monodiferric phosphate 

 Fe203(P205)3 or monodialu- 

 minum phosphate AI2O3P2O5 1.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and one of 6 in 1. 



(2) Add 2.0 g. of nitrogen in the form of 

 NaN03 and 20.0 g. of test material 

 to (1). 



(3) Distribute in Fernbach flasks. 

 Sterilization: Sterilize in the autoclave. 

 Use: To study the cycle of the phosphate 



ion in the soil. Bact. fiuorescens lique- 

 faciens and Bact. Hartlebi were the 

 organisms used. 



Added nutrients: The author added 20.0 g. 



of any carbohydrate. 

 Reference: Stoklasa (1911 p. 479). 



106. Lantzsch's Basal Nitrate Solution 



Constituents : 



1. Water (tap) 100.0 cc. 



2. K2HPO4 (0.01%) = 0.01 g. 



3. NaN03 (0.01%) = 0.01 g. 



4. MgS04 trace 



5. FeCls trace 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 100.0 g. tap 

 water or in 29 parts tap water + 80 

 parts distilled water. 



(2) Place in an atmosphere of one of the 

 test materials. 



Sterilization: Not specified. 



Use : To study utilization of gaseous carbon 



compounds. 

 Added nutrients: The author prepared the 



various atmospheres of test materials as 



follows: 



(a) Decompose 0.6 g. lead formate with 

 H2SO4, in a 2500.0 cc. flask. 



(b) 2.0 cc. of a 4.0% formalin solution in a 

 2000.0 cc. flask. 



(c) 0.125 g. CH3COOH in 2500 cc. flask. 



(d) 0.1 g. acetone in a 2000.0 cc. flask. 



(e) 0.2 cc. butyric acid in 5 liter flask. 



(f) 0.12 cc. of benzol or xylol in 2000.0 

 cc. flask. 



Reference: Lantzsch (1922 p. 310). 



SUBGROUP I-B. SECTION 8 



Inorganic liquid media of known compo- 

 sition; nitrogen supplied as nitrates; com- 

 plete nutrient solutions. 

 Ai. Containing oxidizable sulphur. 

 Bi. Sulphur added as elementary sulphur. 



Beijerinck's Sulphur Nitrate Solution 107 

 B2. Sulphur added as thiosulphate. 

 Lieske's Thiosulphate Nitrate Solu- 

 tion ^(Gehring) 108 



Trautwein's Thiosulphate Nitrate 



Solution 109 



Nathansohn's Thiosulphate Nitrate 



Solution (Trautwein) 110 



A2. Not containing oxidizable sulphur. 

 Beijerinck's Ammonium Nitrate 



Solution Ill 



Nabokich and Leedeff's Hydrogen 



Nitrate Solution 112 



Bokorny's Calcium Nitrate Solution. 113 



