CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



35 



107. Beijerinck's Sulphur Nitrate Solution 



Constituents : 



1. Ditch water or dis- 

 tilled water 100.0 cc. 



2. Sulphur (powder) 10.0 g. 



3. KNO3 0.05 g. 



4. NasCOa 0.02 g. 



5. CaCOs 2.0 g. 



6. K2HPO4 0.02 g. 



7. MgCl. 0.0 or 0.01 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Completely fill a well stoppered flask 

 with (1). 



Sterilization: Not specified. 



Use: To study denitrification with sulphur 

 o.xidation by Thiobacillus denitrificans . 

 The author found that the nitrate was 

 reduced, and the sulphur o.xidized to sul- 

 phate, with the liberation of nitrogen gas. 



Reference: Beijerinck (1903-4 p. 597). 



108. Lieske's Thiosulphate Nitrate Solution 



(Gehring) 



Constituents : 



1. Distilled water 100.0 cc. 



2. NaaSaOs 0.5 g. 



3. KxNOs 0.5 g. 



4. NaHCOs 0.1 g. 



5. K2HPO4 0.02 g. 



6. MgCl2 0.01 g. 



7. Fe.Cle trace 



8. CaCl2 trace 



Prepj ration : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Distribute in test tubes 45 cm. high. 

 Sterilization: Not specified. 



Use: Isolation and cultivation of denitri- 

 fying thiosulphate bacteria, Thiobacillus 

 denitrificans. 



Reference: Gehring (1914 p. 405), Traut- 

 wein (1921 p. 518). 



109. Trautwein's Thiosulphate Nitrate 

 Solution 



Constituents: 



1. Distilled water 100.0 cc. 



2. Na2S203 0.5 g. 



3. KNO3 0.5 g. 



4. NaaCOa 0.1 g. 



5. K2HPO4 0.02 g. 



6. MgClj 0.01 g. 



7. KCl trace 



8. Iron chloride trace 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6, 7 and 8 in 1. 



(2) Distribute in tall glass cylinders. 



(3) Inoculate with waste water filtrate. 

 Sterilization: Not specified. 



Use: Enrichment and isolation of thionic 

 bacteria (Omeliansky). A slight opal- 

 escence is formed after 6 days. After a 

 milky turbid culture results, a snow white 

 membrane is formed on the surface. 



Variants : The author suggested the follow- 

 ing solution: 



1. Distilled water 1000.0 cc. 



2. MgCl2 0.01 g. 



3. KNO3 0.1 g. 



4. Na2HP04 0.02 g. 



5. Na2S203 0.2 g. 



6. NaHCOa 0.1 g. 



Reference: Trautwein (1920 p. 515) (1921 



p. 515). 



110. Nathansohn's Tliiosulphate Nitrate 

 Solution (Trautwein) 



Constituents : 



1. Distilled water 100.0 cc. 



2. NaCl 3.0 g. 



3. MgCl. 0.25 g. 



4. KNO3 0.1 g. 



5. Na2HP04 0.05 g. 



6. NaoSoOs 0.2 or 0.1 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 



Sterilization: Not specified. 



Use: Cultivation of thionic bacteria (Ome- 

 liansky). 



Reference: Trautwein (1921 p. 518). 



111. Beijerinck's Ammonium Nitrate 

 Solution 

 Constituents : 



1. Water (tap) 100.0 cc. 



2. NH4NO3 0.2 g. 



3. Potassium bi-phosphate 0.05 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of gonidia of algae. 



Variants: Beijerinck used 0.02 g. NH4NO3 

 and 0.02 g. K2HPO4 in 100.0 cc. of tap 

 water to cultivate Chlorophijcae, Chloro- 

 coccum and Cyanophycee. 



References: Beijerinck (1893 p. 371), Beij- 

 erinck (1901 p. 563). 



