36 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



112. Nabokoch and Lebedeff's Hydrogen 



Nitrate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Na,HP04 0.5 g. 



3. KNO, 2.0 g. 



4. MgSO, 0.2 g. 



5. NaHCOa 1.0 g. 



6. FbsCU some 



7. Hydrogen 

 Preparation : 



(1) Dissolve 2, 3, 4, 5 and 6 in 1. 



(2) The reaction is slightly alkaline 

 (about 0.050 g. H2SO4 to 100.0 cc. of 

 solution). 



(3) Distribute in 100 or 150.0 cc. lots in 

 vacuum flasks with side tubes. 



(4) Inoculate. 



(5) Remove all the air by means of an 

 oil pump. 



(6) Replace the air by a mi.xture of oxygen 

 and hydrogen ("Knallgase") contain- 

 ing carbonic acid. 



Sterilization: Method not given. 



Use: To study the oxidation of hydrogen 



by microorganisms. 

 Reference: Nabokich and LebedefT (1907 



p. 352), Lohnis (1913 p. 106). 



113. Bokomy's Calcium Nitrate Solution 



Constituents : 



1. Water 100.0 cc. 



2. Ca(N03)2 0.1 g. 



3. MgS04 (crystalline) 0.02 g. 



4. KH2PO4 0.02 g. 



5. Iron chloride trace 



6. KCl 0.0 or 0.05 g. 



Preparation: (1) Dissolve 2, 3, 4, 5 and 6 



in 1. 



Sterilization : Not specified. 



Use: To study the metals required for the 

 growth of Spirogyra majuscula. It was 

 found that potassium was necessary. 



Reference: Bokorny (1912 p. 125). 



SUBGROUP I-C 



Liquid Media with One or More 

 Constituents Organic 



The liquid media in which one or more 

 constituents are organic may be divided 

 conveniently into sections based upon the 

 character of the nitrogen and carbon sources 



supplied. The sections recognized may be 

 differentiated as follows: 



Key to the sections of Subgroup I-C 



Ai. Chemical composition of all constit- 

 uents known. 

 Bi. Nitrogen inorganic, carbon organic. 

 Ci. Nitrogen present as free or elementary 

 nitrogen only. . Section 1 (Med. 114-190) 

 C2. Nitrogen present as ammonium salts. 



Section 2 (Med. 191-323) 

 Cj. Nitrogen present as nitritesj. 



Section 3 (Med. 324-327) 

 C4. Nitrogen present as nitrates. 



Section 4 (Med. 328-365) 

 B2. Nitrogen organic. 

 Ci. Nitrogen present as amino acids. 



Section 5 (Med. 368-479) 



C2. Organic nitrogen other than amino 



acids present . . . Section 6 (Med. 480-515) 



A2. Chemical composition of one or more 



constituents not definitely known. 

 Bi.* Containing digests. 

 Ci.* Containing commercial peptones or 



digests. 

 Di. Additional constituents, if any, in- 

 organic Section 7 (Med. 516-555) 



D2. Containing additional organic constit- 

 uents. 

 El. Chemical composition of all additional 



materials known. 

 Fi. No additional nitrogen compounds 



added. 

 Gi. Basal solutions. Employed with the 

 addition of various nutrients. 



Section 8 (Med. 556-588) 

 B2. Complete nutrient solutions. 



Section 9 (Med. 589-647) 

 F2. At least one additional nitrogenous 

 constituent. 



Section 10 (xMed. 648-683) 

 E2. Composition of one or more additional 



materials not definitely known. 

 Fi. Additional organic material exclusively 

 of plant origin 



Section 11 (Med. 684-712) 

 F2. Containing additional materials of 

 animal origin; plant derivatives may also 

 be present. 

 G:. Not containing extracts or infusion. 



Section 12 (Med. 713-747) 

 G2. Containing extracts or infusion. 



Section 13 (Med. 748-977) 



See page 37 for B2 and C2. 



