CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



39 



(d) 5.0 g. cellulose +0.2 g. dextrose in 



1000.0 cc. solution 



(e) 2.5 g. cellulose +0.2 g. dextrose in 



500.0 cc. solution 



(f) 5.0 g. cellulose +0.2 g. dextrose in 



500.0 cc. solution 



(g) 2.5 g. cellulose +0.2 g. dextrose in 



500.0 cc. solution 

 (h) 5.0 g. cellulose + 0.2 g. mannite in 

 1000.0 cc. solution 

 Reference: Pringsheim (1909 p. 303), (1910 

 p. 224). 



115. Waksman and Joffe's Basal Glucose 



Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Glucose 30.0 g. 



Preparation : 



(1) Dissolve 2 in 1. 



(2) Add 2.0 g. of one of the added nu- 

 trients. 



(3) Tube in 10 to 12 cc. lots. 

 Sterilization: Sterilize at 15 pounds for 15 



minutes. 



Use: To study reaction changes in Actino- 

 mycetes metabolism. 



Added nutrients: The authors added 2.0 g. 

 of one of the following nitrogen sources: 

 (NH4)2S04 (pH of solution = 5.8) 

 (XHOsCOa (pH of solution = 6.8) 

 Urea (pH of solution = 7.4) 



Reference: Waksman and Joffe (1920 p. 39). 



116. Buchanan's Sucrose Solution 



Constituents : 



1. Water (tap) 1000.0 cc. 



2. Sucrose (2.0%) 20.0 g. 



Preparation : 



(1) Prepare a 2.0% saccharose solution 

 in tap water. 



(2) Distribute in 500.0 cc. lots in liter 



Sterilization: Method not given. 



Use: Development of gum produced by 

 Bacillus radicicola. The author found 

 that 1.0% N/1 malic acid or citric acid 

 was sufficient to completely inhibit 

 growth. 1.0% N/1 NaOH stimulated 

 growth and gum production, while 2.0% 

 prevented growth. To isolate the gum 

 add 100.0 cc. of 95% alcohol, to the 2 

 week old culture, filtering the white pre- 

 cipitate, wash in alcohol and dissolve in 



distilled water. Repeat the precipita- 

 tion 5 times. Finally dry the precipi- 

 tate. Saccharose may be used in higher 

 concentrations. 

 Reference: Buchanan (1909 p. 371). 



117. Waksman and Joflfe's Basal Glycerol 

 Solution 



Constituents : 



1. Water to make 1000.0 cc. 



2. Glycerol 30.0 g. 



Preparation : 



(1) Dissolve 2 in 1. 



(2) Add one of the test materials (added 

 nutrients) to (1). When using casein 

 and egg albumin, dissolve casein and 

 egg albumin in N/10 NaOH. In using 

 fibrin, add fibrin to each tube. 



(3) Solutions vary in pH = 7.2 to 7.7, 

 urea pH = 8.0. 



(4) Tube in 10-12 cc. lots. 

 Sterilization: Autoclave for 15 minutes at 



15 pounds pressure. 

 Use: To study change in reaction in 



Actinomycetes metabolism. 

 Added nutrients: The authors added 5.0 g. 

 of one of the following nitrogenous com- 

 pounds: 



fibrin leucin 



casein glycocoll 



egg albumin urea 



peptone acetamide 5.0 g. 



asparagin 

 Variants: The authors used 3.0 g. of glyc- 

 erol and added 2.0 g. of NaNOa, NaNOs, 

 or (NH4)2S04 as inorganic nitrogen 

 sources. 

 Reference: Waksman and JofTe (1920 pp. 

 36-37). 



118. Buchanan's Glycerol Solution I 



Constituents: 



1. Water (tap) 1000.0 cc. 



2. Glycerol 5.0 cc. 



Preparation: (1) Dissolve 2 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of Monascus purpurens 



from silage. 

 Variants: The author used a 10.0, 20.0 or 



40.0% glycerol solution instead of 5.0%. 



Growth in the 5.0 and 10.0% solution, but 



little or no growth in the 20.0 or 40.0% 



solutions. 

 Reference: Buchanan (1910 p. 102). 



