40 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



119. Beijerinck and van Delden's Phosphate 

 Glucose Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Glucose 20.0 g. 



3. K2HPO4 0.5 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not given. 



Use: To study nitrogen assimilation by 

 chroococcum. 



Variants: Stoklasa prepared a solution of 

 25.0 g. of glucose and 1.5 g. K2HPO4 in 

 1000.0 cc. of Moldau river water. He 

 studied the relation between the assim- 

 ilation of phosphoric anhydride and 

 nitrogen by azotobacter. It was deter- 

 mined that 1.0 g. of phosphoric acid 

 anhydride and 2 to 2.3 g. elementary N 

 were assimilated. 



Reference: Beijerinck and van Delden 

 (1902 p. 8), Stoklasa (1911 p. 461). 



120. Beijerinck's Phosphate Sucrose 

 Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. Sucrose 20.0 g. 



3. K2HPO4 0.2 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 



Use: Growth of Bacillus radicicola. 

 Reference: Beijerinck (1901 p. 575). 



121. Beijerinck's Phosphate Mannitol 

 Solution 

 Constituents : 



1. Water 1000.0 cc. 



2. Mannitol 20.0 g. 



3. K2HPO4 0.2 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Reaction is slightly alkaline. 



(3) Inoculate with 0.1 to 0.2 g. earth. 

 Sterilization: Not specified. 



Use: Used for the enrichment of Azotobac- 

 ter chroococcum and for nitrogen assim- 

 ilation studies. 



Variants : 



(a) Beijerinck specified the use of ditch 

 water. 



(b) Jacobitz specified the use of distilled 

 water and studied nitrogen fixation 

 hy Bacillus ellenhachensis a (Caron). 



(c) Christensen specified distilled water 



and suggested that CaCOs might be 

 added if desired. 



(d) Percival specified the use of tap 

 water. 



(e) Beijerinck and van Delden used 0.5 g. 

 K2HPO4 instead of 0.2 g. K0HPO4. 



(f ) Stoklasa specified Moldau river water 

 and used 0.5 g. potassium phosphate 

 instead of 0.2 g. K2HPO4. 



Reference: Beijerinck (1901 pp. 568, 578), 

 Jacobitz (1903 p. 101), Christensen (1907 

 p. 110), Percival (1920 p. 179), Beijerinck 

 and Van Delden (1902 p. 8), Stoklasa 

 (1908 p. 489). 



122. van Delden's Basal Lactate Solution 



Constituents : 



1. Water (ditch) 1000.0 cc. 



2. K2HPO4 0.2 g. 



3. Sodium lactate 1.0 g. 



Preparation : 



(1) Dissolve 2 and 3 in 1. 



(2) Additional nutrients may be added 

 as indicated below. 



Sterilization : Not specified. 



Use : Cultivation and enrichment of Micro- 



spira desulfuricans . Used also to study 



sulphate reduction. 

 Variants : 



(a) The author used 0.5 g. sodium lactate. 



(b) The author used 0.5 g. K2HPO4 and 

 1.0 g. of calcium lactate. 



Added nutrients: 



(a) The author did not specify the use of 

 ditch water, and added (NH4)2S04 

 andO.Og. MgS04-7H20. 



(b) The author did not specify the use of 

 ditch water and added 0.2 g. glycocoU 

 andO.Og. MgS04-7H20. 



(c) The author used 0.5 g. sodium lactate 

 with 0.5 g. K2HPO4 and added 1.0 g. 

 asparagin and 0.0, 0.3, 0.6, 0.9, or 

 1.2 g. gypsum. 



(d) The author specified the use of tap 

 water, used 0.5 g. sodium lactate with 

 0.5 K2HPO4, added NH4CI and 1.2 g. 

 gypsum. 



(e) The author specified the use of tap 

 water, used 0.5 g. of sodium lactate 

 with 0.5 g. K2HPO4 and added 0.5 g. 

 asparagin. 



(f) The author specified the use of tap 

 water, used 0.5 g. of sodium lactate 

 with 0.5 g. K2HPO4 added 1.0 g. 



