CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



41 



asparagin and 1.0 g. gypsum or 4.0 g. 



MgS04-7H20. 

 (g) The author specified the use of tap 



water, used 0.5 g. of sodium lactate 



with 0.5 g. K2HPO4 added 2.5 g. 



asparagin and 4.0 g. MgS04-7H20. 

 (h) The author specified the use of tap 



water, used 10.0 g. sodium lactate, 



added 1.0 g. of asparagin and 2.0 or 



5.0 g. of MgS04-7H20. 

 Reference: van Delden (1903-04 p. 85). 



123. Beijerinck and van Delden's Acetate 



Solution 

 Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 0.5 g. 



3. Sodium acetate 5.0 g. 



Preparation: (1) Dissolve 2 and 3 in 1. 

 Sterilization: Not specified. 



Use: Cultivation of chroococcum and 

 granulobacter. The medium was in- 

 oculated with 20.0 g. of fresh earth. 



Reference: Beijerinck and van Delden 

 (1902 p. 29). 



124. Gage's Glucose Solution 



Constituents: 



1. Water 1000.0 cc. 



2. Glucose 5.0 g. 



3. MgS04 0.25 g. 



4. CaCl2 0.01 g. 



5. Iron sulphate solution 1 drop 



Preparation: (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use : To study the fixation of nitrogen by 

 Pseudomonas radicicola and nitroso bac- 

 teria. It was found that nitrites and 

 possibly nitrates were produced by Bacil- 

 lus radicicola in this medium. 



Reference: Gage (1910 p. 34). 



125. Stoklasa's Phosphorus Free Glucose 



Salt Solution 

 Constituents : 



1. Distilled water 1000.0 cc. 



2. d-glucose 25.0 g. 



3. K2SO4 1.0 g. 



4. MgCl2 0.5 g. 



5. Iron sulphate 0.1 g. 



Preparation : (1) Dissolve 2, 3, 4 and 5 in 1. 

 Sterilization: Not specified. 



Use : To study the role of phosphorus in the 

 soil. It was found that growth of nitro- 



gen fi.xing organisms was very poor in this 

 solution. Phosphorus is required for 

 luxuriant and rapid development. 

 Variants : 



(a) The author suggested the addition 

 of 1.0 g. of nitrogen in the form of 

 (NH4)2S04or NaNO.,. 



(b) The author suggested the addition of 

 0.1 g. of aluminum sulphate and 1.0 g. 

 of one of the following phosphorus 

 compounds: 



dicalcium phosphate CaHP04-2H20. 

 Monodiferric phosphate Fe203(P205)3. 

 Monodialuminum phosphate AI2O3- 



(P205)2-8H20. 



Tricalcium phosphate Ca3(P04)2-2H20. 

 Ditriferric phosphate (Fe03)4(P205)5- 



3H2O. 

 Triferric phosphate Fe203P205 + 4H2O. 

 Trialuminum phosphate AloOj-PjOs + 



4H2O. 



(c) The author used variant (b) and sub- 

 stituted arabniose for d-glucose. 



(d) The author added aluminum sulphate 

 and the equivalent of 0.2 g. of phos- 

 phoric anhydride in the form of 

 monomagnesium phosphate, mono- 

 dialuminum phosphate, monodiferric 

 phosphate or trimagnesium phos- 

 phate to the original solution. 



References: Stoklasa (1911 pp. 492, 441, 

 490). 



126. Gerlach and Vogel's Basal Glucose 



Salt Solution 

 Constituents: 



1. Distilled water 1000.0 cc. 



2. Glucose 5.0 g. 



3. Potassium phosphate 0.5 g. 



4. MgS04 0.3 g. 



5. NaCl 0.5 g. 



6. Na.COs 0.5 g. 



7. FeS04 0.2 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Add one of the nitrogen sources 

 listed in added nutrients. 



(3) Adjustment of reaction not given. 

 Sterilization : Method not specified. When 



utilizing urea as nitrogen source sterilize 

 the urea at 100°C. dry heat. 

 Use: Cultivation of albumin formers from 

 soil and stable manure. These organ- 

 isms grow very well on agar, gelatin and 



