42 



CULTURE MEDIA FOR CULTIVATION OF MICROORGANISMS 



bouillon which contain 0.3% NaNOs. 

 The nitrate in this medium is changed to 

 nitrite after a few days and then quanti- 

 tively to insoluble albuminous materials. 

 Ammonia salts and urea also are suitable 

 nitrogen sources for albumin production 

 by these organisms. Albumin produc- 

 tion using urea however goes on very 

 slowly. 



Added nutrients : The author employed the 

 following nitrogen sources: 



NaNOa (c.p. Merck) 3.0 g 



(NH4)2S04 3.0 g 



NH4NO3 2.0 g 



NH4CO3 2.0 g 



urea 20.0 g 



Reference : Gerlach and Vogel (1901 p. 612) 



127. Henneberg's Basal Glucose Disodium 



Phosphate Solution 



Constituents : 



1. Water 1000.0 cc. 



2. Na2HP04 3.0 g. 



3. MgS04 2.0 g. 



4. NaCl 2.0 g. 



5. Glucose 20.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4 and 5 in 1. 



(2) Add one of the added nutrients 

 (amounts not specified). 



(3) Adjustment of reaction not given. 

 Sterilization: Method not given. 



Use: To study the food requirements for 

 acetic acid organisms, B. Pasteurianum, 

 B. oxydans, B. aceti and B. Kiltzingi- 

 anum. The author found that KNO 3 was 

 the poorest source of nitrogen and 

 (NH4)2S04 was the best. 



Added nutrients : The author employed one 

 of the following salts or carbon sources: 

 KNO3 asparagin 



(NH4)2S04 peptone 



ammonium tartrate 



Reference: Henneberg (1898 p. 18). 



128. Beijerinck's Glucose Salt Solution 



Constituents : 



1. Water 1000.0 cc. 



2. KH2PO4 0.5 g. 



3. MgS04 0.5 g. 



4. Glucose 10.0 g. 



Preparation: (1) Dissolve 2, 3 and 4 in 1. 

 Sterilization: Not specified. 



Use: Growth oi Streptothrix chromogena. 



Variants : 



(a) Peklo used the solution to grow plant 

 actinomyces. He specified that the 

 solution might be neutralized by the 

 addition of 0.5 g. NaoCOs. 



(b) Malenkovic used 100.0 g. glucose, 

 1.0 g. K2HPO4 and added 2.0 g. of 

 KNO3or2.0g.NH4H2PO4. He culti- 

 vated coniophora cerebello (Corti- 

 cium putaneum) and reported 

 NH4H2PO4 a better nitrogen source. 



Reference: Beijerinck (1900 p. 7), Peklo 

 (1910 p. 514). Malenkovic (1906 

 p. 412). 



129. Kisch's Basal Glucose Salt Solution 



Constituents: 



1. Water 1000.0 cc. 



2. K2HPO4 1.0 g. 



3. MgS04 0.5 g. 



4. NaCl 0.02 g. 



5. FeS04 trace 



6. Calcium phosphate trace 



7. Glucose 10.0 g. 



Preparation : 



(1) Dissolve 2, 3, 4, 5, 6 and 7 in 1. 



(2) Add Na2C03 until the reaction is 

 slightly alkaline to litmus. 



(3) Add one of the nitrogen sources listed 

 under added nutrients to (2). 



Sterilization: Not specified. 



Use: To study the nitrogen requirements 



of members of the colon-typhoid group. 

 Added nutrients: The author added one 



of the following nitrogen sources. 



(a) KNO3 0.29 g. 



Bad. paratyphi B. shows growth. 

 Bad. enter itidis Gartner little 

 growth. 



(b) KNO2 2.43 g. 



No growth. 



(c) Urea 8.6 g. 



Bad. coli and Bad. enteritidis Gart- 

 ner show some growth. 



(d) Urea 8.6 g. 



KNO2 2.4 g. 



B. coli shows growth. 



(e) (NH4)2S04 1.9 g. 



B. paratyphi shows good growth. 

 B. coZt shows good growth. B. enteri- 

 tidis Gartner, and dysentery bacillus 

 show good growth. 



(f) NH4H2PO4 1.42 g. 



Bad. paratyphi shows good growth. 



